Device Operation Clause Samples

Device Operation. All fluids were injected into the microfluidic device by loading into individual syringes (Gastight, ▇▇▇▇▇▇▇▇) driven by syringe pumps (PHD 22/2000, Harvard Apparatus). Protein solutions or bead suspensions were mixed with a solu- tion of substrate (500 μM fluorescein-di-β-D-galactopyranoside, FDG, Invitrogen) in PBS buffer (137 mM NaCl, 2.7 mM KCl, 8 mM Na2HPO4, and 2 mM KH2PO4, pH 7.4, Ambion) containing 0.1% v/v Tween-20 (Sigma) in the microfluidic device prior to droplet generation at the flow-focusing nozzle. Water droplets are formed in fluorinated oil (HFE-7500, Novec, 3M) previously mixed with a surfactant (5% w/w, Methods) to generate dro- plets stably and prevent their coalescence. × + Fluorescence Image Acquisition and Analysis. Fluorescence images were obtained using an inverted microscope (IX71, Olympus) operated in epifluorescence mode using a mercury lamp (U-H100HG, Olympus) as an excitation source. The micro- fluidic devices were illuminated, and the emitted light was collected using the same objective (UPLSAPO 40 2, Olympus); excitation light was passed through a neutral density filter (25% transmission, Olympus) and only during image acquisi- tion, in order to minimize photobleaching. Excitation light was spectrally filtered and separated from fluorescence emission using two mirror sets (excitation 475 ( 17 nm/emission 530 ( 22 nm, and excitation 559 ( 17 nm/emission 630 ( 35 nm, Thorlabs); green- and red-fluorescence micrographs were col- lected sequentially using a motorized filter cube (IX2-RFACA-1-5, Olympus) to alternate between the two colors. Images were acquired using an EMCCD camera (Xion , Andor Technologies) with exposure times of 0.1 and 1 s for red and green fluorescence, respectively. Image analysis was performed using custom soft- ware written in LabView, which calculated the fluorescence intensity of femtodroplets by integrating the brightness of all the component pixels of each droplet. × ∼ Measurement of Femtodroplet-Generation Frequencies. A high- precision optical setup was used to measure the frequency of droplet formation. Briefly, the 488 nm beam of a diode laser (Spectra-Physics) was directed to the back port of an inverted microscope (Eclipse TE2000-U, Nikon), where it was reflected by a dichroic mirror and focused 2 μm above the cover slide into the flow-focusing nozzle of the microfluidic device, using an oil- immersion objective (Apochromat 60 , NA 1.40, Nikon). Fluo- rescence was collected by the same objectiv...
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Device Operation. The ▇▇▇▇▇▇ Monitoring System is comprised of an ORCA designed and constructed monitoring instrument that measures flow speed, flow direction, water depth and water temperature. The ▇▇▇▇▇▇ Monitoring system is integrated with a YSI EXO2 sonde with probes measuring ph/ORP, Conductivity/Temperature, Optical DO, Turbidity, total algae that includes chlorophyll and blue green algae sensors and fDOM. Additionally, the ▇▇▇▇▇▇ is integrated with a NuLab nutrient monitoring system that monitors nitrate, nitrite and ortho-phosphate by utilizing wet chemistry which is not affected by bromides, water color changes and other contaminants that affect optical nutrient sensors. The integrated package is coupled to an ORCA designed and constructed communications system that reports to the ORCA designed database at 4-hour intervals. The complete system is solar powered and utilizes a battery backup for times when sunlight is not available. Each deployment site will be visited no less than once per month at which time YSI measurements will be verified using a YSI handheld sonde identical to the deployed instrument measuring salinity/conductivity/temperature, dissolved oxygen, pH/ORP, fDOM, total algae, and turbidity. The handheld sonde will be calibrated and verified with applicable portions of DEP SOPs FT 1000 (field testing general), FT 1100 (pH), FT 1300 (salinity), FT 1500 (DO) and FT 1900 (field continuous monitoring) prior to the site visit, and then verified after the monthly site visits. Verifications for pH and salinity will be conducted with standards that bracket the sample readings from the field. If post- sampling verifications do not meet acceptance criteria listed, associated sample data will be qualified with “J” (estimated). Any deployed instrument found out of calibration will be returned to the lab for calibration and check before redeployment. Calibration checks will be recorded in the ORCA database prior to and after recalibration. At each monthly site visit grab samples will be obtained by ORCA team members ▇▇▇▇▇ ▇▇▇▇▇ and ▇▇▇▇▇▇ ▇▇▇▇▇▇▇. Grab samples will be sent overnight to PACE Analytical Services, Inc. for comparability assessment of Nitrate/Nitrite, Orthophosphate and Chlorophyll a. At each monthly maintenance check, reagents for the NuLab nutrient monitoring device will be replenished and a grab sample will be obtained for verification.
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Related to Device Operation

  • Commercial Operation (i) On or before December 31, 2021, Interconnection Customer must demonstrate commercial operation of all generating units. Demonstrating commercial operation includes achieving Initial Operation in accordance with Section 1.4 of Appendix 2 to this ISA and making commercial sales or use of energy, as well as, if applicable, obtaining capacity qualification in accordance with the requirements of the Reliability Assurance Agreement Among Load Serving Entities in the PJM Region.

  • Co-operation Each Party acknowledges that this ESA must be approved by the Department and agree that they shall use Commercially Reasonable efforts to cooperate in seeking to secure such approval.

  • Safe Operations Notwithstanding any other provision of this Agreement, an NTO may take, or cause to be taken, such action with respect to the operation of its facilities as it deems necessary to maintain Safe Operations. To ensure Safe Operations, the local operating rules of the ITO(s) shall govern the connection and disconnection of generation with NTO transmission facilities. Safe Operations include the application and enforcement of rules, procedures and protocols that are intended to ensure the safety of personnel operating or performing work or tests on transmission facilities.

  • Business Operations Company will provide all necessary equipment, personnel and other appurtenances necessary to conduct its operations. Company will conduct its business operations hereunder in a lawful, orderly and proper manner, considering the nature of such operation, so as not to unreasonably annoy, disturb, endanger or be offensive to others at or near the Premises or elsewhere on the Airport.

  • Wireless Operator Data In addition to Section 20 (Information Authorization) of the General Terms, you acknowledge that we or Zelle® may use information on file with your wireless operator to further verify your identity and to protect against or prevent actual or potential fraud or unauthorized use of the Service. By using the Zelle® Payment Service, you authorize your wireless operator (AT&T, Sprint, T-Mobile, US Cellular, Verizon, or any other branded wireless operator) to disclose your mobile number, name, address, email, network status, customer type, customer role, billing type, mobile device identifiers (IMSI and IMEI) and other subscriber status and device details, if available, to us or our Service Providers solely to allow verification of your identity and to compare information you have provided to us or to Zelle® with your wireless operator account profile information for the duration of our business relationship. See Zelle®’s Privacy Policy at ▇▇▇▇▇://▇▇▇.▇▇▇▇▇▇▇▇.▇▇▇/privacy-policy for how it treats your data. Please review our Privacy Policy in order to better understand our commitment to maintaining your privacy, as well as our use and disclosure of your information.