Razorback sucker Clause Samples

Razorback sucker. IV.A.4.a.(1) Middle Green FWS-FR Ongoing X X X X X X IV.A.4.a.(2) Upper Colorado River. FWS-FR Ongoing X X X X X X IV.A.4.b. Bonytail UDWR/CDOW Ongoing X X X X X X IV.A.4.c. Humpback chub. IV.A.4.c.

Razorback sucker. IV.A.4.a.(1) Middle Green FWS-FR Ongoing X X X X X X IV.A.4.a.(2) Upper Colorado River. FWS-FR Ongoing X X X X X X IV.A.4.b. Bonytail UDWR/CPW Ongoing X X X X X X IV.A.4.c. Humpback chub. Ad hoc group recommends collecting fin clips from adult humpback chub to determine level of genetic introgression (relates to broodstock development). IV.A.4.c.

Razorback sucker. IV.A.4.a.(1) Middle Green (including spawning bar at RM 311.5) FWS-FR Ongoing X X X X X X X IV.A.4.a.(2) Upper Colorado River. FWS-FR Ongoing X X X X X X X IV.A.4.b. Bonytail UDWR Ongoing X X X X X X X IV.A.4.c. Humpback chub. IV.A.4.c.

Razorback sucker. IV.A.4.a. (2) Upper Colorado River IV.A.4.b. Bonytail IV.B. Conduct annual fish propagation activities

Razorback sucker. IV.A.4.a.(1) Middle Green FWS-FR Ongoing X X X X X X X X Maintain genetic refugia for each of the species. IV.A.4.a.(2) Upper Colorado River. FWS-FR Ongoing X X X X X X X X Maintain genetic refugia for each of the species. IV.A.4.b. Bonytail UDWR/CPW Ongoing X X X X X X X X Maintain genetic refugia for each of the species. IV.A.4.c. Humpback chub. A draft report on the genetics of Gila spp., including humpback chub, indicates historical hybridization (not anthropogenic) occurred between humpback chub and roundtail chub in Black Rocks. The authors identified two management units in the upper basin: Deso-Cataract and Black Rocks- Westwater. Authors did not recommend separate broodstocks, rather both management units be represented in a single Upper Basin broodstock. IV.A.4.c.(1) Black Rocks Canyon. FWS-FR Ongoing X X X X X X X X Maintain genetic refugia for each of the species. Eighteen adult HBC from Black Rocks are being held at Horsethief Canyon Native Fish Facility. As was the case in 2015, these fish spawned in the hatchery ponds again in 2016. See IV.A.4.c. IV.A.4.c.(2) Westwater Canyon. UDWR Ongoing X X X X X X X X Maintain genetic refugia for each of the species. IV.A.4.c.

Razorback sucker. IV.A.4.a.(1) Middle Green FWS-FR Ongoing X X X X X X X Maintain genetic refugia for each of the species. Broodstock are currently maintained and in active use at Ouray National Fish Hatchery - Randlett. IV.A.4.a.(2) Upper Colorado River. FWS-FR Ongoing X X X X X X X Maintain genetic refugia for each of the species. Broodstock are currently maintained and in active use at Horsethief Canyon Native Fish Facility. IV.A.4.b. Bonytail UDWR/CPW Ongoing X X X X X X X Maintain genetic refugia for each of the species. Bonytail broodstock are currently maintained and in active use at Southwest Native Aquatic Resources and Recovery Center (Southwest Native ARRC). IV.A.4.c. Humpback chub. A draft report on the genetics of Gila spp. (▇▇▇▇, in prep), including humpback chub, indicates historical hybridization (not anthropogenic) occurred between humpback chub and roundtail chub in Black Rocks. The authors identified two management units in the upper basin: Deso-Cataract and Black Rocks- Westwater. Authors did not recommend separate broodstocks, rather both management units be represented in a single Upper Basin broodstock. Report will be finalized upon inclusion of Westwater samples. IV.A.4.c.(1) Black Rocks Canyon. FWS-FR Ongoing X X X X X X X Maintain genetic refugia for each of the species. Ten adult humpback chub from Black Rocks were brought into captivity. A total of 28 adult HBC from Black Rocks are being held at Horsethief Canyon Native Fish Facility. See IV.A.4.c. IV.A.4.c.(2) Westwater Canyon. UDWR Ongoing X X X X X X X Maintain genetic refugia for each of the species. IV.A.4.c.

Razorback sucker. The razorback sucker is a large-bodied, long-lived catostomid sucker endemic to the ▇▇▇▇▇▇▇▇ ▇▇▇▇▇ ▇▇▇▇▇ ▇▇ ▇▇▇ ▇▇▇▇▇▇▇▇▇▇▇▇ ▇▇▇▇▇▇ ▇▇▇▇▇▇, including the mainstem Colorado River and major tributaries. Adult razorback suckers are characterized by the presence of a distinct dorsal bony keel and thickened, foreshortened caudal rays thought to be adaptations to historic flow and current conditions in Colorado River mainstem and tributary habitats. Adult fish can reach 1 meter (m) in length and a weight of up to 6 kilograms (kg) although lengths of 400-740 millimeters (mm) are typical. The razorback sucker was historically common and widely distributed from Mexico to major lower and upper Colorado River basin tributaries including the Gila and Salt rivers in Arizona and the Green, Yampa and San ▇▇▇▇ ▇▇▇▇▇▇ in Utah, Colorado, Wyoming and New Mexico. Lower Colorado River basin populations exhibited significant declines in numbers and distribution beginning in 1935 with the creation of Lake ▇▇▇▇, and subsequently, other mainstem Colorado River reservoirs. Declines in upper basin adult fish captures have accelerated in the last 20 years indicating an ongoing loss of upper basin populations in most known habitats (USFWS 1998). An absence of recruitment, related to both habitat alterations and the increased presence of introduced fish species, is assumed to be the primary cause of population declines on a range wide basis but the great longevity of adult fish (<40 years, ▇▇▇▇▇▇▇▇ and ▇▇▇▇▇▇▇▇ 1987) has allowed the retention of old-aged, reproducing

Razorback sucker. Of the 45 individuals captured, 43 received PIT tags, two did not as they were considered too small to tag at time of capture. Catch rates for age-1 Razorback Sucker fall between 95% confidence intervals (CI) of 0.62 and 0.33 fish per hour of electrofishing with an average of 0.47 fish per hour (Figure 2.) CPUE (Fish/Hr) 0 RBS ALL CPM Colorado Pikeminnow In addition to Razorback Sucker, Colorado Pikeminnow were captured as well. To reduce potential handling stress on juvenile Colorado Pikeminnow (▇▇▇▇▇ et al. 2018), only adult and sub adult Colorado Pikeminnow (>400mm TL) were originally targeted for netting. However, the 2019 spring sampling trip was during a time period with cooler water temperatures, and handling smaller size classes (<300mm TL) would be less stressful to the fish compared to summer months when the smaller size classes were normally captured. A number of fish were captured for purposes of other studies that fell between the 300mm TL and 400mm TL. A total to 274 individual Colorado Pikeminnow were captured during this trip with no within trip recaptures. Sizes of fish ranged from 152mm to 751mm TL with weights ranging from 23 to 4000g. The highest frequency of fish was between 210 and 299mm TL, encompassing 219 fish (76.6%) with a peak at fish between 280 and 289mm TL (Figure 3). Two recruiting sub adult size class fish (400-449mm TL) and eight adult size class fish (>450mm TL) were captured throughout this trip as well (Figure 3). Of the 274 individuals captured, 21 (7.66%) had a PIT tag, the other 253 individuals were implanted with one prior to release. Of the 21 fish that had a PIT tag, one was a recruiting sub adult (429mm TL), and eight were adult size class fish. Individual Count

Razorback sucker. IV.A.4.a.(1) Middle Green FWS-FR Ongoing X X X X IV.A.4.a.(2) Upper Colorado River. FWS-FR Ongoing X X X X X X IV.A.4.b. Bonytail UDWR/CPW Ongoing X X X X X X Federal and state hatcheries were evaluated during 2015 for the opportunity to house a backup broodstock for bonytail; the NFH facility at ▇▇▇▇, NM was selected in the spring of 2016. IV.A.4.