Sample Preparation Clause Samples
The Sample Preparation clause defines the requirements and procedures for preparing samples for analysis, testing, or evaluation. It typically outlines the methods to be used, the standards to be followed, and any specific handling or labeling instructions necessary to ensure consistency and reliability. By establishing clear guidelines for how samples should be prepared, this clause helps prevent errors, ensures comparability of results, and maintains the integrity of the testing process.
Sample Preparation. Users are responsible for preparing their own samples; however, the Facility Manager must approve the prepared samples (see #6) and may disallow samples that are not stable and could damage the instrument. The Facility Manager will train Members in sample preparation and may provide assistance in special cases, but in general Members are responsible for their own samples.
Sample Preparation a. Laboratory Molded Specimens - Use cylindrical specimens that have been compacted using the gyratory compactor (AASHTO T 312). Specimen diameter must be 6 inches (150 mm) and a specimen height must be 4.5 inches +/- 0.2 inches (115 +/- 5 mm).
Sample Preparation. ]” shall mean, individually and collectively, the major [...***...]
Sample Preparation. (1) Sample to be processed should correlate with stage and site to be treated.
(2) Cut one 5 µM section, mount on glass slide.
(3) Cut four 10 µM sections, mount on four separate regular glass slides. Glass slides should be regular glass, uncoated and uncharged. Do not bake slides and do not use coverslip. Slides must contain a sufficient quantity of tumor tissue to be successfully microdissected.
(4) Send above referenced samples or specimens by overnight delivery in approved slide holder, placed in a bubble-lined envelope and in a Federal Express Diagnostic Specimen Envelope, to the laboratory designated by Response at Response's direction. Portions of this Exhibit were omitted and have been filed separately with the Secretary of the Commission pursuant to the Company’s application requesting confidential treatment under Rule 406 of the Securities Act.
Sample Preparation. A. Serum
B. Urine
Sample Preparation. Human calmodulin (CaM) was expressed and purified in Escherichia coli BL21(DE3) cells, as described F ▇▇▇▇▇://▇▇▇.▇▇▇/10.1021/jacs.2c02201 previously.9 Two site-directed mutations at A17C and A128C in the NTD and CTD domains of CaM, respectively, were introduced as sites of attachment for the nitroxide spin-labels.9 Full deuteration was achieved by growing the bacteria in deuterated minimal medium, with U-[12C/2H]-glucose as the sole carbon source. R1 nitroxide labeling was carried out with S-(1-oxyl-2,2,5,5-tetramethyl-2,5-dihydro-1H- pyrrol-3-yl)methyl methanesulfonothioate (MTSL; Toronto Research Chemicals), as described previously.9 The purity of A17C-R1/ A128C-R1 nitroxide-labeled calmodulin was verified by mass spectrometry. Solutions for DEER EPR comprised 50 μM CaM (A17C-R1/ A128-R1), 8 mM CaCl2, 100 mM NaCl, 25 mM d-HEPES pH 6.4, and 20% (v/v) d8-glycerol, placed in 1.0 mm inner diameter (1.2 mm outer diameter) quarts EPR tubes (VitoCom). Freezing of the DEER samples was carried out using three different approaches: slow rate freezing (∼40 s) by placing the EPR tube in a −80 °C freezer;43 intermediate rate freezing (∼1.5 s) by directly placing the EPR tube in liquid N2;43 and rapid freeze-quenching (∼0.5 ms) by spraying a high- speed jet onto a spinning copper plate cooled to 77 K, as described previously.18,45 Q-Band DEER. Pulsed EPR data were collected at Q-band (33.8 GHz) and 50 K on a Bruker E-580 spectrometer equipped with a 150 W traveling-wave tube amplifier, a model ER5107D2 resonator, and a cryofree cooling unit, as described previously.52 DEER experiments were acquired using a conventional four-pulse sequence (Figure S1).53 The observer and ▇▇▇▇▇ pump pulses were separated by ca. 90 MHz, with the observer π/2 and π pulses set to 12 and 24 ns, respectively, and the ▇▇▇▇▇ π pulse to 10 ns. The pump frequency was centered at the Q-band nitroxide spectrum located at +40 MHz from the center of the resonator frequency. The τ1 value of 350 ns for the first echo period time was incremented eight times in 16 ns steps to average 2H modulation; the position of the ▇▇▇▇▇ pump pulse was incremented in steps of Δt = 10 ns. The bandwidth of the overcoupled resonator was 120 MHz. All DEER echo curves were acquired for tmax = 7.5 μs, with the exception of the DEER echo curve for τ2 < 7.5 μs, where tmax was set to the value of τ2. DEER data were recorded with values of the dipolar evolution time T (=2τ2) set to 10, 15, 20, 25, 30, and 35 μs. Measurement t...
Sample Preparation. 1. Add 1 µL Activator to 100 µL patient sample or control. Mix and allow to sit at room temperature for 10 minutes.
2. If testing fewer than 10 samples, remove one Applicator Blade Assembly from the packaging. If testing 11 to 20 samples, remove two disposable Applicator Blade Assemblies from the packaging.
3. Place the Applicator Blade into the vertical slot 6 in the Applicator Assembly. If using two Applicator Blades, place them into the vertical slots numbered 6 and 12.
Sample Preparation. 1. Prepare hemolysates of patient specimens and controls as instructed in the "Specimen Preparation" section.
2. Remove one Disposable Applicator Blade from the packaging. If testing more than 10 samples, remove two Applicator Blades from the packaging. Additional blades must be ordered (Cat. No. 3450) if testing 11 to 20 samples per gel. Remove the protective guard from the blades by gently bending the protective piece back and forth until it breaks free.
3. Place the Applicator Blade into the vertical slots numbered 8 in the Applicator Assembly. If using two Applicator Blades, place them into the vertical slots numbered 8 and B. NOTE: The Applicator Blade will only fit into the slots in the Applicator Assembly one way; do not try to force the Applicator Blade into the slots.
4. Place an Applicator Blade Weight on top of each blade assembly.
5. Slide the Disposable Sample Cups into the top row (numbered 1 to 10) of the appropriate cup tray. If testing more than 10 samples, place cups into both rows.
6. Pipette 17 µL of the patient or control hemolysate into cups 1 to 5 and 6 to 10. If testing more than 10 samples, pipette sample into cups 11 to 15 and 16 to 20. Cover the tray until ready to use.
Sample Preparation. I. The total sample collected without exclusion of anything other than foreign materials viz. metallic pieces, wood, bricks, concrete chips etc., shall constitute the gross sample for the lot.
Sample Preparation. 1. Remove one Disposable Applicator Blade from the packaging. If testing more than 10 samples, remove two Applicator Blades from the packaging.
2. Place the Applicator Blade into the vertical slot numbered 6 in the Applicator Assembly. If using two Applicator Blades, place them into the vertical slots numbered 6 and 12. NOTE: The Applicator Blade will only fit into the slots one way; do not try to force the Applicator Blades into the slots.