Common use of SOLUBILITY TESTS Clause in Contracts

SOLUBILITY TESTS. Solubility tests were carried out by saturating NADES with an excess of the tested compound in a bottle with a cap, stirring at 40 oC for 2 h and leaving to rest for 3 h for precipitation (centrifuging 20 min for DNA and gluten after dissolving for 2h). Triplicate samples of the resulting solution were diluted with water. The diluted solutions were analyzed with HPLC-UV at wavelength of 360 nm for rutin, 370 nm for quercetin, 272 nm for cinnamic acid, 517 nm for carthamin, 472 nm for 1,8-dihydroxyanthaquinone, and quantified with a UV/Vis spectrophotometer at 217 nm for ginkgolide B, 228 nm for taxol, 260 nm for DNA, and 595 nm for gluten with Bradford method. Solubility of starch was performed as follows: a known amount of starch was placed in a glass vial with a cap and 5 mL of NADES were added. The vial was vortexed, then loosely capped, placed in a microwave oven and repeatedly heated with 5-10 s pulses at full power till the liquid boiled. Between pulses, the vial was removed, and 1/10 of the original amount of the sample was added, vortexed and replaced in the oven if fully soluble. If not soluble, the sample amount was reduced and the above steps were repeated till a cloudy solution became clear when boiled (▇▇▇▇▇▇▇▇▇ et al., 2002).

SOLUBILITY TESTS. Solubility tests were carried out by saturating NADES with an excess of the tested compound in a bottle with a cap, stirring at 40 oC for 2 h and leaving to rest for 3 h for precipitation (centrifuging 20 min for DNA and gluten after dissolving for 2h). Triplicate samples of the resulting solution were diluted with water. The diluted solutions were analyzed with HPLC-UV at wavelength of 360 nm for rutin, 370 nm for quercetin, 272 nm for cinnamic acid, 517 nm for carthamin, 472 nm for 1,8-dihydroxyanthaquinone, and quantified with a UV/Vis spectrophotometer at 217 nm for ginkgolide B, 228 nm for taxol, 260 nm for DNA, and 595 nm for gluten with Bradford method. Solubility of starch was performed as follows: a known amount of starch was placed in a glass vial with a cap and 5 mL of NADES were added. The vial was vortexed, then loosely capped, placed in a microwave oven and repeatedly heated with 5-10 s pulses at full power till the liquid boiled. Between pulses, the vial was removed, and 1/10 of the original amount of the sample was added, vortexed and replaced in the oven if fully soluble. If not soluble, the sample amount was reduced and the above steps were repeated till a cloudy solution became clear when boiled (▇▇▇▇▇▇▇▇▇ Swatloski et al., 2002).