Exhibit 10.10
STRATEGIC ALLIANCE AGREEMENT
This Strategic Alliance Agreement (hereinafter "Agreement"), by and
between Geneva Proteomics, Inc., a corporation organized under the laws of
Delaware ("GeneProt"), Bruker Daltonics Inc., a corporation organized under the
laws of Delaware ("BDAL") and Bruker Daltonik GmbH, a company organized under
the laws of Germany ("BDAL Germany") is made as of September 12, 2000
("Effective Date").
I. RECITALS AND OVERVIEW
A. GeneProt is in the business of large-scale industrial
proteomics and BDAL is a developer and provider of innovative life science tools
based on mass spectrometry;
B. BDAL Germany desires to sell to GeneProt, and GeneProt desires
to purchase from BDAL Germany, certain Products (defined below) in the
quantities and on the terms as set forth in this Agreement;
C. GeneProt wishes to receive engineering support relating to the
Products from BDAL and BDAL Germany;
D. GeneProt and BDAL desire to enter into a related transaction
relating to ownership of equity in each other; and
E. GeneProt and BDAL desire to formalize the ownership of certain
technologies that the parties, either individually or jointly, may develop and
other aspects of a long-term relationship.
II. AGREEMENT
NOW, THEREFORE, the parties agree as follows:
1. DEFINITIONS. Defined terms used in this Agreement shall have the
following meanings:
1.1 "Consumables" means the items listed under the heading
"Consumables" on Exhibit A hereto.
1.2 "DOCUMENTATION" means user manuals for the Products and
all other written materials provided by BDAL with respect to the use and
maintenance of the Products.
1.3 "PRICE LIST" means BDAL Germany's standard commercial fee
schedule or other price notification releases furnished by BDAL Germany
generally to its customers, a current copy of which is attached hereto as
EXHIBIT D.
1.4 "PRODUCTS" means, collectively, the Systems acquired by
GeneProt under this Agreement, the Consumables and the other items listed on
Exhibit A as more particularly described on Exhibit D, which GeneProt has the
option to purchase under this Agreement. The term "Product" also includes any
enhancement or improvement of a Product that is made generally available by
BDAL, that is substantially similar to such Product, and is marketed under the
same product number and nomenclature as such Product.
CONFIDENTIAL TREATMENT HAS BEEN REQUESTED AS TO CERTAIN PORTIONS OF THIS
EXHIBIT, WHICH PORTIONS HAVE BEEN OMITTED AND REPLACED WITH [**] AND FILED
SEPARATELY WITH THE COMMISSION.
1.5 "SERVICES" means the software/applications research and
development support described in EXHIBIT C.
1.6 "SYSTEMS" means BDAL Germany's Reflex III MALDI-TOF and
esquire3000 ion trap mass spectrometry systems acquired under this Agreement as
more particularly described on the Price List attached as EXHIBIT D HERETO.
2. TERMS AND CONDITIONS OF PRODUCT SALE.
2.1 SALE AND PURCHASE OF PRODUCTS. Pursuant to the terms and
conditions of this Agreement, BDAL Germany shall sell to GeneProt, and GeneProt
shall purchase from BDAL Germany, the Systems in the quantities and at the
prices set forth on Exhibit A hereto and in the manner described in the General
Terms and Conditions set forth on Exhibit B hereto (the "General Terms and
Conditions"). In addition, pursuant to the terms and conditions of this
Agreement, BDAL Germany shall make the Consumables available for purchase in the
manner and with the discounts from the then current Price List as described in
the General Terms and Conditions. The parties acknowledge and agree that BDAL
Germany shall appoint an operational point of contact for purposes of this
Agreement.
2.2 PROVISION OF SERVICES. In connection with the sale of the
Products pursuant to ▇▇▇▇▇▇▇ ▇.▇, ▇▇▇▇ ▇▇▇▇▇▇▇ will provide the Services in the
manner described in the General Terms and Conditions.
2.3 PRICING AND PAYMENT. Unless otherwise agreed upon by the
parties, BDAL Germany will invoice GeneProt for payment upon the date of BDAL
Germany's delivery of the applicable Products to the shipping carrier. All
payments shall be made in European denominations in accordance with the General
Terms and Conditions. All amounts shown in the Exhibits hereto are in the Euro.
2.4 TAXES AND OTHER CHARGES. Purchase prices, and any other
payments made hereunder with respect to the Products, do not include and are net
of any foreign or domestic governmental taxes or charges of any kind that may be
applicable to the use or sale of the Products.
2.5 TRADE-IN OPTION. Subject to the terms and conditions of
this Agreement, BDAL and BDAL Germany hereby grant to GeneProt the Trade-In
Option more particularly described on the General Terms and Conditions.
2.6 DESTRUCTION OF PRODUCTS. In the event that all or
substantially all of the Products are destroyed due to fire to other calamity
while the Products are in the possession of GeneProt, upon the placement of a
new order for such Products by GeneProt with BDAL Germany, BDAL Germany shall
make it a top priority of BDAL Germany's production line to manufacture
replacement Products for GeneProt.
3. TERMS AND CONDITIONS OF STOCK PURCHASE
3.1 PURCHASE AND SALE OF GENEPROT STOCK. Subject to the
terms and conditions of this ▇▇▇▇▇▇▇ ▇, ▇▇▇▇ agrees to purchase and GeneProt
agrees to sell Nine Hundred Nine Thousand and Ninety One (909,091) shares of
GeneProt's Series B Preferred Stock (the "GeneProt Stock") at a per share
purchase price not to exceed [**] per share. BDAL shall pay such purchase
price by delivering Seven Million Dollars ($7,000,000) in cash to GeneProt
and 79,218 shares of fully paid and nonassessable shares of BDAL's common
stock.
2
[**] Indicates that information has been omitted and filed separately with
the Commission pursuant to a request for confidential treatment.
3.2 TIMING AND TERMS OF PURCHASE. The parties agree that BDAL
shall purchase the GeneProt Stock as part of the Series B Preferred Stock round
currently being undertaken by GeneProt (the "Series B Round"). Such investment
shall be pursuant to the stock purchase, investor rights and other agreements,
documents and instruments used by GeneProt for the other investors in the Series
B Round. Such agreements, documents and instruments shall be no less favorable
to BDAL than the agreements, documents and instruments which are most favorable
to any investor in the Series B Round. GeneProt agrees that it shall not sell,
transfer or otherwise dispose of any BDAL stock received hereunder for a period
of twenty four (24) months after receipt of such stock. The timing of BDAL's
investment shall occur simultaneously with the closing of the first $50,000,000
(including BDAL's investment). However, if such investment has not closed on or
before November 30, 2000 through no fault of BDAL, BDAL shall have the right not
to close on such investment thereafter.
4. DELIVERY; TITLE; ACCEPTANCE.
4.1 SHIPPING DATES. BDAL Germany shall deliver the Products on
their respective delivery dates as set forth in the General Terms and
Conditions. In the event GeneProt determines that it requires a change in the
actual scheduled delivery date, GeneProt will notify BDAL Germany as soon as is
reasonably practicable following such determination and BDAL Germany shall use
its commercially reasonable efforts to comply with the requested schedule
change.
4.2 TITLE. Title to the Products shall pass to GeneProt upon
Acceptance (defined below) of the Products by GeneProt. Risk of loss or damage
to the Products, other than due to an act or omission of BDAL or BDAL Germany,
shall pass to GeneProt upon delivery.
4.3 ACCEPTANCE.
(a) For purposes of this Agreement, "Acceptance" of a
Product by GeneProt shall be deemed to have occurred upon completion of the
installation of such Product by BDAL Germany and the execution of an Acceptance
Certificate by GeneProt, a form of which is attached hereto as EXHIBIT E (THE
"ACCEPTANCE CERTIFICATE").
(b) GeneProt may reject any Product which is not
conforming with the warranties set forth in Section 5. In order to reject a
Product, GeneProt must (i) give notice to BDAL Germany of GeneProt's intent to
reject the shipment within thirty (30) days of receipt together with a written
indication of the reasons for such rejections, and (ii) as promptly as
reasonably practicable thereafter, provide BDAL Germany with notice of final
rejection and the full basis therefor. After notice of intent to reject is
given, GeneProt shall cooperate with BDAL Germany in determining whether
rejection is necessary or justified. If no such notice of intent to reject is
timely received, GeneProt shall be deemed to have accepted such delivery of
Products; provided, however, that such acceptance shall in no way limit any
remedies GeneProt may have with respect to the warranties set forth in Section
4.
5. WARRANTIES.
5.1 PRODUCTS. BDAL Germany and BDAL warrant to GeneProt
that the Products delivered to GeneProt will be free from defects in material
and workmanship for [**] months from the date of Acceptance of such Products,
(the "Warranty Period") and shall perform substantially in accordance with
BDAL Germany's published technical specifications, which are set forth in the
Acceptance Certificate, and that such Products shall perform without defect
when installed in GeneProt's proteomics factory.
3
[**] Indicates that information has been omitted and filed separately with
the Commission pursuant to a request for confidential treatment.
5.2 SERVICES. BDAL Germany and BDAL warrant to GeneProt that
during the Warranty Period,
(a) In performing the Services, BDAL Germany shall
comply with all applicable laws of local, state and federal governments and
agencies.
(b) All Services shall be performed by qualified
personnel at a level of professional performance standard within the industry in
which the Services are provided.
5.3 INFRINGEMENT. BDAL and BDAL Germany warrant to GeneProt
that they are not aware of any patent, copyright, trade secret or trademark of a
third party which has been used without permission to manufacture or complete
any Product or which has been incorporated therein without permission.
5.4 COMPLIANCE WITH LAW. BDAL Germany and BDAL warrant to
GeneProt that, at the time of shipment, all Products sold to GeneProt hereunder
shall, when required, be in compliance with applicable laws and regulations.
5.5 LIMITATIONS. EXCEPT AS SET FORTH IN THIS AGREEEMNT, BDAL
AND BDAL GERMANY MAKE NO OTHER WARRANTIES WITH RESPECT TO THE PRODUCTS OR ANY
SERVICES AND DISCLAIMS ALL OTHER WARRANTIES, EXPRESS OR IMPLIED, INCLUDING
WITHOUT LIMITATION, WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR
PURPOSE.
5.6 WARRANTY SERVICES. With respect to each Product, during
the applicable Warranty Period, BDAL Germany will provide to GeneProt the
maintenance, repair and return services described in Exhibit F. If considered by
BDAL Germany staff to be advisable to improve the reliability of the Products,
components will be replaced or modified without additional cost, and replaced
components will become the property of BDAL Germany, all during the Warranty
Period. In addition, software bug fixes will be provided during the Warranty
Period free of charge.
5.7 SITE GUIDE. The Site Guides for the Products are included
in Exhibit E.
6. GENEPROT COVENANTS. GeneProt agrees:
6.1 REVERSE ENGINEERING. Not to (and not to allow any third
parties to) (a) disassemble, decompile or otherwise reverse engineer the
Products.
6.2 NO REMOVAL OF MARKS. To include, and not alter, obscure or
remove, any logo, trademark, copyright notice or other ▇▇▇▇ used or claimed by
BDAL or BDAL Germany, or any markings, colors or other insignia which are
contained in or affixed to the Product at the time of shipment.
6.3 ACCESS. To provide BDAL Germany, or its designee, with all
physical and electronic access to the Products reasonably necessary in
GeneProt's opinion to permit BDAL Germany to perform the Services and the
warranty services.
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7. PROVISION OF PERSONNEL.
7.1 SERVICES PERSONNEL. During the Warranty Period, BDAL
Germany shall provide four (4) man years of its full-time employees to perform
the Services (the "BDAL Employees"). Each man year shall be approximately 1800
hours of direct labor. The BDAL Employees shall provide the Services at such
times and locations as shall be reasonably requested by GeneProt. The BDAL
Employees shall be available at all times during the normal business hours of
GeneProt to provide the Services and Improvements by telephone or in person. The
Services shall be performed in collaboration with GeneProt.
7.2 SITE MANAGER. During the Warranty Period, BDAL Germany
shall provide a site manager as described in the General Terms and Conditions.
7.3 STATUS OF PERSONNEL. The parties acknowledge and agree
that the BDAL Employees and any site manager shall not be deemed to be employees
of GeneProt and shall remain employees of BDAL Germany. GeneProt shall have no
obligation to provide any employee related benefits, including, without
limitation, health, pension plan, social security, life or disability insurance,
sick leave, vacation leave, work permits, personal liability or separate payment
to such personnel. BDAL Germany agrees to inform such personnel of the
foregoing.
8. OWNERSHIP AND LICENSE.
8.1 As between the parties, GeneProt exclusively shall have
all right, title and interest (including all patent rights, copyrights, trade
secret rights and all other intellectual property rights throughout the world)
(the "Intellectual Property Rights") in any inventions, methods, instruments,
consumables, software, works-of-authorship, ideas or information made or
conceived or reduced to practice ("Inventions") by GeneProt through its use of
the Products or Systems under this Agreement.
8.2 Except for the licenses granted by BDAL and BDAL Germany
to GeneProt hereunder, as between the parties, BDAL and BDAL Germany exclusively
shall have all Intellectual Property Rights in any Inventions by BDAL or BDAL
Germany through its use of the Products or Systems under this Agreement.
8.3 With respect to Inventions for which employees or
consultants of both parties are joint inventors or authors, each party will
equally and jointly own the Intellectual Property Rights with respect to the
joint Inventions with the right to unilaterally fully exploit or nonexclusively
license such rights without accounting or further consent.
BDAL and BDAL Germany hereby grant to GeneProt a worldwide,
nonexclusive, nontransferable, perpetual, irrevocable license to use, modify
and otherwise exploit any BDAL Inventions related to the large-scale analysis
of expressed proteins according to the business model of GeneProt as
described in the business plan of GeneProt dated May 2000.
9. TERMINATION OF AGREEMENT.
9.1 TERMINATION BY EITHER PARTY. This Agreement may be
terminated by a party for cause immediately upon the occurrence of any of the
following events:
(a) If the other party ceases to do business, or
otherwise terminates its business operations without a successor; or
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(b) If the other party shall seek protection under
any bankruptcy, receivership, trust deed, creditors arrangement, composition or
comparable proceeding, or if any such proceeding is instituted against the other
(and not dismissed within 60 days); or
(c) If the other party breaches any material
provision of this Agreement and, for any breach other than a payment breach,
fails to cure such breach within thirty (30) days of written notice describing
the breach.
9.2 NOT SOLE REMEDY. Termination is not the sole remedy under
the Agreement and, whether or not termination is effected, all other remedies
remain available and shall be cumulative. Upon a default hereunder, each party
reserves to itself all remedies available to it in equity, under the Uniform
Commercial Code, or under any other applicable law, notice of the non-defaulting
party's exercise thereof being expressly waived by the defaulting party.
9.3 EFFECT OF TERMINATION. Any accrued rights to payment,
remedies for breach and those rights and obligations set forth in Sections
2,3,8, 10, 11, 12 and 15 shall survive any termination or expiration of this
Agreement.
10. CONFIDENTIAL INFORMATION. As used in this Agreement, "Confidential
Information" will mean the terms of this Agreement and any confidential or
proprietary information that is marked or otherwise designated as "Confidential"
or is clearly by its nature confidential and disclosed in any medium by one
party to the other. Except as required by law or regulation or expressly and
unambiguously allowed herein, the receiving party will hold in confidence and
not use or disclose any Confidential Information of the disclosing party and
shall similarly bind its employees in writing. Without limiting the foregoing,
each party agrees to employ with regard to the Confidential Information of the
other party procedures no less restrictive than the strictest procedures used by
it to protect its own confidential and proprietary information. These
nondisclosure obligations will not apply to information which the receiving
party can document has entered the public domain without any action or inaction
by it. Each party will promptly report to the other party any actual or
suspected violation of the terms of this Section 9, and will take all reasonable
further steps requested by the other party to prevent, control or remedy any
such violation. Each party will, upon the termination or expiration of the
Agreement or the request of the other party at any time, return to the
disclosing party all tangible manifestations of Confidential Information
received by it pursuant to the Agreement (and all copies and reproductions
thereof). Notwithstanding this Section 9, any confidentiality or non-disclosure
agreement(s) previously entered into between GeneProt and BDAL shall continue in
full force and effect in accordance with their terms.
11. INFRINGEMENT. BDAL and BDAL Germany agree to indemnify, defend and
hold harmless GeneProt and its officers, directors, shareholders, affiliates,
employees and agents against any and damages, including reasonable expenses,
arising from a third party claim that GeneProt's use of a Product in accordance
with the terms of this Agreement infringes or misappropriates any patent,
copyright, trade secret or other proprietary or intellectual property right of
any such third party; provided that GeneProt provides prompt notice to BDAL and
BDAL Germany of such claim and the opportunity to assume sole control of the
defense or settlement of such claim. In the event that any Product is held to
constitute such an infringement and the use of such Product is enjoined, BDAL
and BDAL Germany by their own election and at its own expense, shall either: (1)
procure for GeneProt the right to continue using such Product; (2) shall modify
the Product so that it becomes non-infringing; or (3) remove the Product and
refund to GeneProt the entire amount paid for such Product and any related
Services. BDAL and BDAL Germany shall have no obligation or liability under this
Section 10 if any infringement claim is based upon portions or components of the
Products (a) that are not supplied by BDAL and ▇▇▇▇
▇
▇▇▇▇▇▇▇, (▇) that are combined with other products, processes or materials
where the alleged infringement relates to such combination, (c) to the extent
GeneProt continues allegedly infringing activity after being notified thereof
or of modifications that would have avoided the alleged infringement or (d)
where GeneProt's use of the Product is incident to an infringement not
resulting primarily from the Product or is not strictly in accordance with
the licenses or restrictions provided in this Agreement.
THE FOREGOING AND SECTION 5.3 HEREOF ARE IN LIEU OF ANY WARRANTIES OF
NON-INFRINGEMENT, WHICH ARE HEREBY DISCLAIMED, AND STATE THE EXCLUSIVE
OBLIGATION AND SOLE LIABILITY OF BDAL AND BDAL GERMANY FOR INFRINGEMENT OR
CLAIMS OF INFRINGEMENT OF INTELLECTUAL PROPERTY RIGHTS.
12. LIMITATION OF LIABILITY. NOTWITHSTANDING ANYTHING ELSE IN THIS
AGREEMENT OR OTHERWISE, GENEPROT WILL NOT BE LIABLE WITH RESPECT TO ANY SUBJECT
MATTER OF THIS AGREEMENT UNDER ANY CONTRACT, NEGLIGENCE, STRICT LIABILITY OR
OTHER LEGAL OR EQUITABLE THEORY FOR AMOUNTS IN EXCESS IN THE AGGREGATE OF THE
AMOUNTS PAID TO BDAL GERMANY HEREUNDER .
13. FORCE MAJEURE. If the performance of any obligation under this
Agreement is interfered with by reason of any circumstances beyond the
reasonable control of the party affected, including, without limitation, fire,
explosion, power failure, acts of God, war, revolution, civil commotion, delays
of the other party in the performance of any of its obligations hereunder,
industry-wide parts shortages, acts of the public enemy, or any law, order,
regulation, ordinance or requirement of any government or legal body, and labor
difficulties, including without limitation, strikes, slowdowns, picketing or
boycotts; then the party affected shall be excused from such performance for a
period equal to the delay resulting from any such causes and such additional
period as may be reasonably necessary to allow the party to resume its
obligations (and the other party shall likewise be excused from performance of
its obligations to the extent such party's obligations relate to the performance
which was interfered with). The party so affected shall make reasonable efforts
to remove such causes of nonperformance.
14. PRINCETON OPTION. Since GeneProt intends to give preferential
treatment to BDAL and BDAL Germany as suppliers for GeneProt's proposed
Princeton, New Jersey facility, for a period of [**] after the date hereof
GeneProt shall have the option to purchase the same number of Systems and
Products for such facility as described in the General Terms and Conditions
at the discounts contained therein from BDAL and BDAL Germany then current
list prices and without the four man years of Services.
15. GENERAL.
15.1 COMPLETE AGREEMENT. This Agreement, together with the
Exhibits attached hereto, and the documents contemplated hereby, contains the
entire agreement of the parties and supersedes all existing agreements and all
previous oral, written, or other communications between them concerning its
subject matter.
15.2 MODIFICATION. No addition or modification of any portion
of this Agreement shall be binding upon GeneProt or BDAL unless such is made in
writing and signed by a duly authorized representative of each party.
15.3 INDEPENDENT CONTRACTOR. The relationship established by
this Agreement between GeneProt and BDAL is that of independent contractors.
BDAL and BDAL Germany have no express or
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[**] Indicates that information has been omitted and filed separately with
the Commission pursuant to a request for confidential treatment.
implied authorization to incur any obligation or commitment on behalf of
GeneProt, unless specifically approved in writing by an authorized GeneProt
official. BDAL and BDAL Germany shall employ and contract their own personnel
and agents and shall be responsible for them and their acts.
15.4 PARAGRAPH AND SECTION HEADINGS. The paragraph and Section
headings contained herein are intended for convenience of reference only and
shall not affect the interpretation of any such provisions.
15.5 VALIDITY. If any provision of this Agreement shall be
held to be invalid, illegal, or unenforceable, the validity, legality or
enforceability of the remaining provisions of this Agreement shall in no way be
affected or impaired. In such event, the parties shall negotiate, in good faith,
a substitute provision which most clearly reflects their original intent for
entering into this Agreement.
15.6 GOVERNING LAW. This Agreement shall be governed by and
construed under the laws of Delaware without reference to conflicts of law
principles and without reference to the United Nations Convention on Contracts
for the International Sale of Goods. The sole jurisdiction and venue for any
action related to the subject matter hereof shall be the local and federal
courts having within their jurisdiction at the location of the defendant's
principal place of business. Both parties consent to the jurisdiction of such
courts and agree that process may be served in the manner provided herein for
giving notices or otherwise as allowed by such local or federal law. In any
action to enforce this Agreement, the prevailing party shall be entitled to
costs and attorneys' fees.
15.7 ENGLISH LANGUAGE. This Agreement is written in the
English language, which shall be controlling for all purposes. No translation of
this Agreement into any other language shall be of any force or effect in the
interpretation of this Agreement or in a determination of the intent of the
parties hereto.
15.8 WAIVER. The waiver by either party hereto of a breach of
any provision of this Agreement shall not be construed as a waiver of any
subsequent breach of the same, or any different, provision.
15.9 NOTICES. All notices remitted under this Agreement shall
be in writing and in the English language. Any and all notices shall be sent by
registered or certified mail, postage prepaid, receipt requested or by confirmed
fax or hand delivery to the other party to the attention and address listed
below, or to such other person(s) or addresses as the parties shall specify by
written notice. Notices will be deemed effective three (3) days after delivery
to a recognized overnight courier service, if so delivered; one (1) business day
after sending if given by facsimile transmission and upon delivery if given by
hand. All notices hereunder shall be remitted to the attention of the:
If to GeneProt, to:
▇▇▇ ▇▇ ▇▇▇▇▇ ▇▇
▇▇▇▇ ▇▇▇▇▇▇
▇▇▇▇▇▇▇▇▇▇▇
Attn: Chief Executive Officer
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If to BDAL, to:
Bruker Daltonics Inc.
▇▇ ▇▇▇▇▇▇▇ ▇▇▇▇
▇▇▇▇▇▇▇▇▇, ▇▇ ▇▇▇▇▇
Attn: ▇▇▇▇▇ ▇. ▇▇▇▇▇▇▇, Ph.D.
President and Chief Executive Officer
If to BDAL Germany, to:
Bruker Daltonik GmbH
▇▇▇▇▇▇▇▇▇▇▇▇▇▇▇▇▇ ▇, ▇-▇▇▇▇▇
▇▇▇▇▇▇, ▇▇▇▇▇▇▇
Attn: ▇▇▇▇ ▇. ▇▇▇▇
15.10 LIMITATION. No action, regardless of form, arising out
of or related to this Agreement, may be brought by either party more than one
(1) year after the cause of action has accrued, except that an action for
non-payment may be brought within one (1) year after the date of last payment.
15.11 NON-SOLICITATION. During the term of this Agreement and
for six (6) months thereafter, without the prior written consent of the other
party, each party agrees not to solicit or initiate contact or communications
with the other party's personnel or contractors for the purpose of hiring or
engaging such personnel as employees or contractors of the contacting party.
15.12 ASSIGNMENT. Neither party may assign or transfer this
Agreement or any rights hereunder (and any attempted assignment will be void)
without the prior written consent of the other party; except for rights to
payment and except to a person or entity who acquires all or substantially all
the assets or business of a party, whether by sale, merger or otherwise.
15.13 ORDER OF PRECEDENCE. Should the terms and conditions of
any Exhibit to this Agreement or the terms of any purchase order delivered by
either party conflict with the terms and conditions of this Agreement, the terms
and conditions of the principal portion of this Agreement shall govern.
[REMAINDER OF PAGE INTENTIONALLY LEFT BLANK]
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IN WITNESS WHEREOF, the parties hereto have executed this Agreement as
of the Effective Date.
GENEVA PROTEOMICS, INC.
By:
-----------------------------------
Name:
-----------------------------------
Title:
-----------------------------------
BRUKER DALTONICS INC.
By:
-----------------------------------
Name:
-----------------------------------
Title:
-----------------------------------
BRUKER DALTONIK GMBH
By:
-----------------------------------
Name:
-----------------------------------
Title:
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[SIGNATURE PAGE TO STRATEGIC ALLIANCE AGREEMENT]
EXHIBIT A
PRICING
---------------------------------------------------------------------------------------------------
INSTRUMENTS LIST [**]
---------------------------------------------------------------------------------------------------
ESQUIRE 9,604,375.00 [**]
REFLEX 2,396,712.00 [**]
SOFTWARE/APPLICATIONS SUPPORT 1,200,000.00 [**]
---------------------------------------------------------------------------------------------------
SUM 13,201,087.00 [**]
---------------------------------------------------------------------------------------------------
---------------------------------------------------------------------------------------------------
ESTIMATE FOR 1YR. OF CONSUMABLES LIST [**]
---------------------------------------------------------------------------------------------------
ESQUIRE 456,395.00 [**]
REFLEX 512,030.00 [**]
---------------------------------------------------------------------------------------------------
SUM 968,425.00 [**]
---------------------------------------------------------------------------------------------------
Note: The 1 Year Consumables are ONLY an estimate and are not included in the
order. The consumables cost estimate do not include HPLC consumables,
solvents, helium gas, microtitre plates, ZIP Tips, data storage, etc. that
are not directly used by the mass spectrometers. For example, 1536 Zip Tips
will be required for each 1536 target prepared. GeneProt is negotiating a
solvent contract separately and is setting up the permanent facilities for
purified water. Each esquire3000 will require approximately 7 atm L/day of
pure Helium gas. The estimate for consumables is a very preliminary draft for
discussion purposes. Until the methods are finalized and tested in the pilot
phase, firm estimates of consumable will be difficult.
[**] Indicates that information has been omitted and filed separately with
the Commission pursuant to a request for confidential treatment.
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SUM: LIST [**] [**] [**]
----------------------------------------------------------------------------------------------------------------------------
TOF 2,239,512.00 [**] [**] [**]
COMPUTER / SW 89,700.00 [**] [**] [**]
TARGETS / MATRIX 67,500.00 [**] [**] [**]
CONSUMABLES ESTIMATE 512,030.00 [**] [**] [**]
----------------------------------------------------------------------------------------------------------------------------
ALL 2,908,742.00 [**] [**] [**]
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ALL (WITHOUT CONSUMABLES EST.) 2,396,712.00 [**] [**] [**]
----------------------------------------------------------------------------------------------------------------------------
---------------------------------------------
TOF [**] TYPE IN %
---------------------------------------------
TOF INSTRUMENTATION [**]
BRUKER MANUFACTURED ACC [**]
EXTERNAL PARTS [**]
---------------------------------------------
----------------------------------------------------------------------------------------------------------------------------
TOF PRICE IN EURO # SUM LIST [**] IN% [**] PRICE
----------------------------------------------------------------------------------------------------------------------------
LINEAR + REFLECTOR 100 278,200.00 [**] [**] [**] [**]
(REFLECTOR, LISTPRICE 52,200)
MAP II 171 45,950.00 [**] [**] [**] [**]
HOOD FOR MAP 175 4,590.00 [**] [**] [**] [**]
2ND YEAR WARRANTY 22,256.00 [**] [**] [**] [**]
3RD YEAR WARRANTY 22,256.00 [**] [**] [**] [**]
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[**] [**]
----------------------------------------------------------------------------------------------------------------------------
----------------------------------------------------------------------------------------------------------------------------
COMPUTER /
SW
----------------------------------------------------------------------------------------------------------------------------
PC FOR OFF-LINE DATA PROCESSING 3,500.00 [**] [**] [**] [**]
COMPUTER FOR MAP INC. WINDOWS NT AND 4,000.00 [**] [**] [**] [**]
MS OFFICE
SUBSTITUTE 21" MONITOR FOR 19" (SUN) 401 1,400.00 [**] [**] [**] [**]
XMASS NT 445 3,100.00 [**] [**] [**] [**]
BIOTOOLS 450 2,950.00 [**] [**] [**] [**]
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[**] [**]
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[**] Indicates that information has been omitted and filed separately with
the Commission pursuant to a request for confidential treatment.
----------------------------------------------------------------------------------------------------------------------------
TARGETS / MATRIX
----------------------------------------------------------------------------------------------------------------------------
#26755 384 TARGET,WITH ANTI-CORROSIVE 253.00 [**] [**] [**] [**]
COATING, GROUND
#72226 ANCHORCHIPTM 200/384 (384 ANCHORS, 414.00 [**] [**] [**] [**]
200[MU]M DIAMETER)
#72227 ANCHORCHIPTM 400/384 (384 ANCHORS, 414.00 [**] [**] [**] [**]
400[MU]M DIAMETER)
#72228 ANCHORCHIPTM 600/384 (384 ANCHORS, 414.00 [**] [**] [**] [**]
600[MU]M DIAMETER)
#72229 ANCHORCHIPTM 800/384 (384 ANCHORS, 414.00 [**] [**] [**] [**]
800[MU]M DIAMETER)
#29414 ANCHORCHIPTM VAR/384 414.00 [**] [**] [**] [**]
384 ANCHORS, 4 DIFF ANCHOR DIAMETER:
96X200[MU]M, 96X400[MU]M, 96X600[MU]M,
96X800[MU]M
#72448 ANCHORCHIPTM 200/1536 (1536 470.00 [**] [**] [**] [**]
ANCHORS, 200[MU]M DIAMETER)
#72449 ANCHORCHIPTM 400/1536 (1536 470.00 [**] [**] [**] [**]
ANCHORS, 400[MU]M DIAMETER)
SANDWICH TARGETS AND ACCESSORIES
#25183 384 TARGET PLATE, STAINLESS 103.00 [**] [**] [**] [**]
STEEL, GROUND
#25184 384 TARGET PLATE, STAINLESS 157.00 [**] [**] [**] [**]
STEEL, POLISHED
# 24983 SCOUT MTP TARGET FRAME 377.00 [**] [**] [**] [**]
# 26475 KEY 62.00 [**] [**] [**] [**]
FOR SANDWICH TARGET ASSEMBLY
PREPURIFIED MATRIX (500MG) 2,500.00 [**] [**] [**] [**]
----------------------------------------------------------------------------------------------------------------------------
[**] [**]
----------------------------------------------------------------------------------------------------------------------------
[**] Indicates that information has been omitted and filed separately with
the Commission pursuant to a request for confidential treatment.
----------------------------------------------------------------------------------------------------------------------------
ESTIMATE OF REFLEX CONSUMABLES PER YEAR
----------------------------------------------------------------------------------------------------------------------------
ANCHORCHIP TARGETS (SPOT TBD) 470.00 [**] [**] [**] [**]
DETECTOR MCP CARTRIDGE 2,250.00 [**] [**] [**] [**]
PREPURIFIED MATRIX (500MG) 2,500.00 [**] [**] [**] [**]
MECHANICAL PUMP OIL 37.00 [**] [**] [**] [**]
----------------------------------------------------------------------------------------------------------------------------
[**] [**]
----------------------------------------------------------------------------------------------------------------------------
[**] Indicates that information has been omitted and filed separately with
the Commission pursuant to a request for confidential treatment.
----------------------------------------------------------------------------------------------------------------------------
SUM: LIST [**] PRICE [**] IN% (CALC.)
----------------------------------------------------------------------------------------------------------------------------
ESQUIRE 8,953,980.00 [**] [**]
COMPUTER 194,000.00 [**] [**]
CONSUMABLES ESTIMATE 456,395.00 [**] [**]
----------------------------------------------------------------------------------------------------------------------------
ALL 9,604,375.00 [**] [**]
----------------------------------------------------------------------------------------------------------------------------
--------------------------------------------
ESQUIRE [**] TYPE IN %
--------------------------------------------
ESQUIRE INSTRUMENTATION [**]
BRUKER MANUFACTURED ACC [**]
EXTERNAL PARTS [**]
--------------------------------------------
----------------------------------------------------------------------------------------------------------------------------
ESQUIRE PRICE IN EURO # SUM LIST [**] IN% [**] PRICE
----------------------------------------------------------------------------------------------------------------------------
ESQUIRE3000 166,000.00 [**] [**] [**] [**]
2ND YEAR WARRANTY 13,280.00 [**] [**] [**] [**]
3RD YEAR WARRANTY 13,280.00 [**] [**] [**] [**]
ON-LINE NANOSPRAY 7,020.00 [**] [**] [**] [**]
21" MONITOR 1,330.00 [**] [**] [**] [**]
----------------------------------------------------------------------------------------------------------------------------
[**] [**]
----------------------------------------------------------------------------------------------------------------------------
----------------------------------------------------------------------------------------------------------------------------
COMPUTER
----------------------------------------------------------------------------------------------------------------------------
DECONVOLUTION 400.00 [**] [**] [**] [**]
BIOTOOLS 2,950.00 [**] [**] [**] [**]
OFFLINE DATAANALYSIS 3,700.00 [**] [**] [**] [**]
MASCOT WORKSTATION 7,500.00 [**] [**] [**] [**]
MASCOT LICENCE 2,750.00 [**] [**] [**] [**]
----------------------------------------------------------------------------------------------------------------------------
[**] [**]
----------------------------------------------------------------------------------------------------------------------------
SUMMARY OF INDIVIDUAL SYSTEM PRICING [**] DUE ON DELIVERY [**] DUE ON ACCEPTANCE NUMBER OF SYSTEMS
ESQUIRE WITH ESI
END PRICE [**] [**] [**] [**]
ESQUIRE WITH ON-LINE NANOSPRAY
END PRICE [**] [**] [**] [**]
ESQUIRE WITH ESI + 21" MONITOR
END PRICE [**] [**] [**] [**]
ESQUIRE WITH ON-LINE
NANOSPRAY + 21" MONITOR
END PRICE [**] [**] [**] [**]
[**] Indicates that information has been omitted and filed separately with
the Commission pursuant to a request for confidential treatment.
-------------------------------------------------------------------------------------------------
ESTIMATE OF ESQUIRE3000 CONSUMABLES PER YEAR
-------------------------------------------------------------------------------------------------
GLASS CAPILLARY 2,150.00 [**] [**] [**] [**]
ESI NEEDLES
(SET OF 2 , #27921) 350.00 [**] [**] [**] [**]
PACKAGE OF 15
NANOSPRAY NEEDLES 300.00 [**] [**] [**] [**]
CHANNEL MULTIPLIER 1,300.00 [**] [**] [**] [**]
CALIBRATION MIXTURE 171.00 [**] [**] [**] [**]
MECHANICAL PUMP OIL 37.00 [**] [**] [**] [**]
-------------------------------------------------------------------------------------------------
[**] [**]
-------------------------------------------------------------------------------------------------
[**] Indicates that information has been omitted and filed separately with
the Commission pursuant to a request for confidential treatment.
EXHIBIT B
GENERAL TERMS AND CONDITIONS
GENERAL TERMS The following General Terms and Conditions are valid
AND CONDITIONS only in connection with the Strategic Alliance
Agreement between GeneProt and BDAL dated as of
September 8, 2000. All terms capitalized, but not
otherwise defined herein shall have the meanings set
forth in the Agreement.
PRICING TERMS AND Prices for all Products are F.O.B., Bremen Germany,
CONDITIONS and do not include import duty, state, local, or any
other taxes but include packing and insurance.
GeneProt will supply with each purchase order a
certificate indicating that the sale is either exempt
from these taxes, or that the buyer will directly
remit any applicable sales/use taxes. This offer
includes equipment purchased under Quotation
#08-11-2000-GPI-3, and the terms and conditions of
this offer supersede Quotation #08-11-2000-GPI-3.
Offer valid until 15 Sept. 2000
QUOTATION This offer number 0/317 includes equipment purchased
#08-11-2000-GPI-3 under Quotation #08-11-2000-GPI-3 and the terms and
conditions of this offer supersed those of Quotation
#08-11-2000-GPI-2.
PAYMENT TERMS Payment for Consumables are invoiced 100% on
delivery.
Payment for each System order must be made as
follows: [**]% of the purchase price of such System
shall be paid with GeneProt's initial order, [**]%
thereof shall be paid within 30 days following
delivery of a System and [**]% shall be made upon
Acceptance of a System. (All payments made as of the
date of this Agreement under Quotation
#08-11-2000-GPI-3 shall be applied toward the
purchase of future Systems.)
Payments not made within the time period set forth
above shall be assessed interest at a rate equal to
the lesser of one and one-half percent (1.5%) per
month or the maximum rate permitted by applicable
law.
[**] LEVEL GeneProt shall receive discounts off the price
of a Product as listed on the Price List in effect in
the country of delivery as of the date of any order
placed under the Agreement. [**] shall be as follows:
[**] for each Reflex MALDI-TOF system,
[**] for each esquire3000 system,
[**] for Consumables, and
[**] for all service not covered by the warranty
provisions of the Agreement or any other provision of
the Agreement.
All Systems purchased under this Agreement include 2
years of extended warranty for which GeneProt is
paying at [**] the original price for each year of
such extensions.
[**] Indicates that information has been omitted and filed separately with
the Commission pursuant to a request for confidential treatment.
TRADE IN OPTION If GeneProt elects to purchase next generation
products similar to the Systems from BDAL within
the first three years after Acceptance of a
System, GeneProt may trade-in such System and
receive a credit against the purchase of a next
generation product as follows:
- Within [**] after Acceptance of a System,
GeneProt would receive a credit equal to [**] of the
System's original purchase price.
- Within [**] after Acceptance of a System,
GeneProt would receive a credit equal to [**] of the
System's original purchase price.
- Within [**] after Acceptance of a System,
GeneProt would receive a credit equal to [**] of the
System's original purchase price.
CONFIGURATION OF [**] esquire3000 Systems with on-line NanoSpray
PURCHASED ESQUIRE3000 [**] esquire3000 Systems with electrospray
SYSTEMS (21"monitors for [**] esquire3000 Systems)
50 copies of BioTools
5 copies each of off-line DA and Deconvolution
(printers for [**] esquire3000 Systems)
CONFIGURATION OF [**] Reflex with linear & reflectron operation, MAP
PURCHASED REFLEX II/8 with hood, MAPII/8 computer, off-line copies of
MALDI-TOF SYSTEMS XMASS and BioTools, and assorted targets.
Printers are to be included with [**] out of [**] of
such Systems.
WARRANTY The warranty described in the Agreement includes both
parts and labor and covers the repair or replacement
of all defective Products, subject to the provisions
of this paragraph. BDAL shall be responsible for all
expenses incurred in performing all warranty
services, including costs related to shipping and
hereby agrees that it shall provide replacement
Products for use by GeneProt such that GeneProt shall
not be without the use of a System for more than 30
days at any one time. For items supplied by not
manufactured by BDAL, the warranty terms of the
manufacturere will be transferred to GeneProt. The
warranty does not cover consumables, pump oil,
routine pump maintenance (e.g. cleaning, oil change,
etc.), MALDI targets, digital media and solvents.
BDAL shall provide one of its employees to GeneProt
for the purpose of acting as a Site Manager upon
completion of the installation and integration
services to be performed by BDAL. The Site Manager
shall be responsible for coordinating and completing
all warranty service on the Products and shall
generally
[**] Indicates that information has been omitted and filed separately with
the Commission pursuant to a request for confidential treatment.
INSTALLATION BDAL shall perform all installation and integration
services necessary to enable the Products to safely
and effectively function in accordance with the
direction of GeneProt. GeneProt shall be obligated to
perform site preparation tasks as described in BDAL's
Site Preparation guide attached to the Agreement as
Exhibit ____.
SERVICE Warranty service of the complete GeneProt proteomics
factory shall be performed under the direction of a
site manager hired and paid by BDAL who shall remain
an employee of BDAL. Such site manager shall perform
such service and shall remain on-site at GeneProt's
proteomics factory during the Warranty Period.
All back-up services will be provided by BDAL
Switzerland. Non-warranty services will be charged as
specified in BDAL's then current Price List minus the
discount set forth herein.
SOFTWARE/ APPLICATIONS BDAL shall provide the equivalent of four man years
R&D AND SUPPORT of expert software, applications and research and
development support from its subsidiary in Bremen,
Germany (BDAL Germany) for the purpose of:
1. Automating and adapting GeneProt's proteomics
factory to meet GeneProt's specifications. In this
process, if GeneProt purchases either a Waters or
Flux HPLC equipment instead of BDAL's standard
▇▇▇▇▇▇▇ ▇▇▇▇, ▇▇▇▇ shall incorporate control of such
equipment into the integrated Proteomics factory
within 90 days after receipt of notice of such
purchase and delivery of the selected pump to BDAL's
Bremen, Germany location.
DELIVERY SCHEDULE BDAL shall deliver the Products purchased under the
Agreement in accordance with the following schedule:
1. On or before [**] BDAL shall deliver [**] Reflex
MALDI-TOF Systems, [**] esquire3000 Systems with
21" monitors and on-line Nanospray and [**]
esquire3000 Systems with 21" monitors and
electrospray. Installation at the customer's site
will commence no later than the [**].
2. On or before [**], BDAL shall deliver [**] Reflex
MALDI-TOF System and [**] esquire3000 Systems with
on-line NanoSpray. Installation of these machines at
the customer's site will commence no later than
the [**].
3. On or before [**], BDAL shall deliver [**] Reflex
MALDI-TOF Systems, [**] esquire3000 Systems with
on-line Nanospray and [**] esquire3000 System with
electrospray. Installation of these machines at
the customer's site will commence no later than
the [**].
4. On or before [**], BDAL shall deliver [**] Reflex
MALDI-TOF System, [**] esquire3000 Systems with
on-line Nanospray and [**] esquire3000 System with
electrospray. Installation of these machines at the
customer's site will commence no later than the
[**].
PRINCETON OPTION GeneProt shall have the option, exercisable within
[**] after Acceptance of all System purchased under
the Agreement for GeneProt's Geneva, Switzerland
factory, to purchase Products in the same quantities
and with the same terms and conditions (including,
without limitation, discounts from the purchase price
of such Products), as set forth in the Agreement.
GeneProt may exercise this option by delivering
written notice to BDAL of its intention at any time
prior to the expiration of the option as described
above. Upon receipt of such notice and a
corresponding order of Products, BDAL covenants that
it will use its best efforts to meet GeneProt's
desired schedule for delivery and installation of
such Products, but in any event BDAL shall deliver
such Systems within [**] after receipt of such
notice and order.
LIABILITY 1. BDAL and BDAL Germany experssly disclaim
responsibility for loss or damage caused by use of
its goods other than in accordance with proper
operating procedures.
2. To the extent that such exclusions are in
compliance with the law, BDAL and BDAL Germany shall
in no event be liable for incidential, consequential
or resulting loss or damage of any kind, however
caused. To the extent that such exclusion is in
compliance with the law, BDAL and BDAL Germany's
liability for damages shall not exceed the payment,
if any received by BDAL and BDAL Germany for the
product or service furnished or to be furnished as
the case may be which is the subject to claim or
dispute. The same applies to any claims of GeneProt
for damages arising from delay or non performance
from positive violation of claims, violation of
duties during contract negotiations or tortious acts.
[**] Indicates that information has been omitted and filed separately with
the Commission pursuant to a request for confidential treatment.
EXHIBIT C
SOFTWARE/APPLICATION RESEARCH AND DEVELOPMENT SUPPORT
1. IMPLEMENTATION AND INTEGRATION. BDAL shall provide expert software,
application and research and development support to GeneProt for its proteomics
factory automation and adaptation to:
integrate [**] into GeneProt's proteomics factory environment
located in Geneva Switzerland as directed by GeneProt;
optimize BDAL's [**] for optimized sensitivity and robust automation
utilizing GeneProt's preferred protocols for first [**] and [**];
integrate the [**] and [**] data output,
appropriately analyzed to GeneProt's preferred analysis protocols
by [**], and use either [**] or GeneProt's preferred [**]
search tool (at GeneProt's discretion) to create
high-throughput protein identification from [**];
integrate either [**], if GeneProt selects either of these systems
instead of the [**]. This integration will be performed within 90
days after receipt of notice of selection given by GeneProt and
delivery of the selected [**] to BDAL's software center in
Bremen, Germany. GeneProt shall obtain the necessary technical
assistance from the pump manufacturer; and
integrate [**] data analysis and interpretation operations as an [**]
for geneprot's selected search engines and sequence databases.
such a [**] and will play an important role in the protein data
management.
[**] Indicates information which has been omitted and filed separately with
the Commission pursuant to a request for confidential treatment.
EXHIBIT D
PRICE LIST / DESCRIPTION OF PRODUCTS
ESQUIRE
3000
----------
HIGH-PERFORMANCE BENCHTOP
HPLC/MS(n) SYSTEM
[PHOTO-DESCRIPTION TO COME]
EXHIBIT D-1
DESCRIPTION AND EUROPEAN PRICE LIST
EXHIBIT D-2
TABLE OF CONTENTS
1. Introduction 4
2. esquire3000 Standard Pricing 6
3. esquire3000 Options 8
3.1. HARDWARE OPTIONS...................................... 8
3.2. SOFTWARE OPTIONS...................................... 10
4. Miscalleneous 11
5. General Terms and Conditions 12
5.1. WARRANTY.............................................. 12
5.2. TERMS AND CONDITIONS.................................. 12
5.3. PAYMENT TERMS......................................... 12
5.4. DELIVERY.............................................. 12
5.5. INSTALLATION.......................................... 12
5.6. SITE PREPARATION...................................... 12
6. Consumables Price List 13
EXHIBIT D-3
INTRODUCTION
Bruker Daltonik's new esquire3000 provides a new analytical flexibility for
the structural characterization and quantitation of complex mixtures in drug
discovery, metabolic profiles, proteomics, combinatorial chemistry and other
applications. This system offers the perfect combination of highest
performance with intuitive software to simplify the setup and interpretation
of HPLC/MS(n) and CE/MS(n) analyses.
The esquire3000 is equipped with the successful Agilent electrospray ion
source, which has proven to be robust and sensitive due to its orthogonal
sprayer design. Technological innovation led to a new level of performance of
Bruker Daltonik's ion trap MS and MSn analyzer. It now provides an unrivaled
mass range of m/z 3000 with monoisotopic resolution and an equally unrivaled
sensitivity in the low pg range.
The intuitive software guarantees for optimized workflow. It features the
unique SmartSuite(TM) for automated system optimization and integrates
applications-oriented tools, e.g. for quantitation and protein analysis.
Most prominent features are:
- Highest sensitivity of all LC-MS/MS instruments (sensitivity 20:1 S/N at
10 pg Reserpin Full Scan MS/MS)
- High mass resolution: 0.3 u FWHM in the m/z range of 50-3000, resulting in
the isotopic resolution for up to (M+3H)(3+) and (M+4H)(4+) ion signals, at
a scan speed of 1650 Da/s.
- Extended Mode with SpeedScan 27.000 Da/s at a mass range of m/z 200 - 6000
with 4u FWHM
EXHIBIT D-4
- Ion Charge Control (ICC) for automated regulation of the ion accumulation
time to provide a wide dynamic range without overloading the trap.
- New SmartSuite -TM- Features:
- SPS -TM- (Smart Parameter Setting) providing optimized parameter settings
for user defined masses.
- SmartCal -TM- Autocalibration/Autotune
- SmartFrag -TM- Ramping during Fragmentation (for easy AutoMS/MS setup
and Library Search)
- EasyTune Page showing only the important parameters
- STS -TM- (Smart Time Segment) Editor setup based on MS data
- SmartRamp -TM- Parameter Ramping for Method development
- Fast scan speed: up to 13,000 Da/s at better than unit resolution for
capturing more than 100 scans across a typical LC peak.
- Subsequent, multiple fragmentation (MS/MS) of analyte molecules up to
MS(11) for structural elucidation.
- New On-Line Nanoelectrospray for capillary LC applications providing
searchable results down to the femtomol range.
- Fast switching between positive and negative ionization
- Divert Valve
Exhibit D-5
ESQUIRE3000 STANDARD PRICING
QTY DESCRIPTION PRICE
--- ---------------------------------------------------------------------- ---------------
ESQUIRE3000 -TM- EURO 166,000.00
NEW ION TRAP LC/MS(N) DESIGNED TO ACCESS A NEW DIMENSION OF
ANALYTICAL FLEXIBILITY AND VERSATILITY FOR STRUCTURAL CHARACTERIZATION
AND QUANTITATION OF COMPLEX MIXTURES IN PROTEOMICS, DRUG
DISCOVERY, METABOLIC PROFILES, COMBINATORIAL CHEMISTRY AND OTHER
LIFE SCIENCE APPLICATIONS.
THE BASIC SYSTEM CONSISTS OF:
1 COMPACT, BENCHTOP ENCLOSURE FOR MASS ANALYZER, ESI SOURCE,
ELECTRONICS AND TURBOPUMPS WITH
- 2 SOFTWARE SELECTABLE MASS RANGES
- STANDARD mass range 50 -3000 u with three scan rates
- NORMAL: 13,000 u/sec, 0.6 u resolution
- ENHANCED: 5550 u/sec, 0.4 u FWHM for isotopic resolution
of up to triply charged ions
- MAXIMUM: 1650 u/sec, 0.3 u FWHM for charge state determination
up to four charges
- HIGH mass range: 200-6000 u at 27,000 Da/s scan rate with
4 u_FWHM
- Same resolution across the full mass range in MS mode and MS(n)
mode(n # 11) for each scan rate
- Highest sensitivity of all ion traps (sensitivity 10 pg MS/MS 20:1
S/N)
- Robust and sensitive Agilent orthogonal ESI source with fast
switching power supply for positive/negative mode
1 ROUGH PUMP (please specify required voltage) INCL.
1 SET OF MANUALS AND REFERENCE CD-ROMS INCL.
1 SYRINGE PUMP (please specify required voltage) INCL.
Exhibit D-6
1 ESQUIRE3000 DATA SYSTEM, CONSISTING OF: INCL.
- HP KAYAK XA 7/500 SI1 with 500 MHz, 10 GB HD, 128 MB RAM,
CD-ROM and CD-Writer
- HP LASERJET 2100 (please specify required voltage)
- HP 17" COLOR MONITOR HP 75
1 SOFTWARE PACKAGE ESQUIRE NT 4.0 INCL.
- Unique SmartSuite TM software features for optimized workflow
- Advanced data processing with automation capability
- New QuantAnalysisTM quantitation package
- LibrarySearch of MS/MS spectra with advanced matching
algorithms
CONSISTING OF:
- BRUKER DALTONICS ESQUIRE NT 4.0 control software package
including
BRUKER DALTONICS POST PROCESSING SOFTWARE DA 2.0 WITH:
- QuantAnalysisTM Module
- LibrarySearchTM Module
- Charge Deconvolution Module
- AGILENT HPLC 2D CHEMSTATION VER A.07.0X
- AGILENT HPLC CHEMSTATION SPECTRAL S/W
1 INSTALLATION INCL.
1 FAMILIARIZATION COURSE (UPON INSTALLATION) INCL.
Exhibit D-7
QTY DESCRIPTION PRICE
------------------------------------------------------------------------------
BRUKER OFF-LINE NANOELECTROSPRAY (PART-# 26871) E 7,320.00
Accessory for off-line electrospray at nanoliter/min
flow rates. Includes 100 coated needles, mount,
positioning controls and video monitoring.
BRUKER ON-LINE NANOELECTROSPRAY (PART-# 29079) E 7,020.00
Accessory for on-line nanoelectrospray for
capillary LC applications (100-500 nl/min).
Includes 20 needles (15mum inner diameter, for
200-500 nl/min) and microscope
----------------------------------------------------
NanoSPRAY was developed in collaboration with
▇. ▇▇▇▇ and ▇. ▇▇▇▇ at the EMBL, and is
exclusively licensed to Bruker for ion traps. Its
ultra-narrow spray tip is loaded with 1 - 10 mul
of sample. Just 2 muul of sample permit measurement
times greater than 30 minutes to study the sample with
MS, MS/MS and MS(n).
----------------------------------------------------
ADDITIONAL NANOELECTROSPRAY NEEDLES 15 MUUM (PACK
OF 20) (PART-# 27364) E 240,00.00
AGILENT APCI SOURCE OPTION (PART-# 72685) E 9,500.00
Atmospheric pressure chemical ionization source
includes APCI nebulizer and vaporizer, spray
chamber, corona discharge needle and calibration
sample. Easy switch-over between ESI and APCI
without breaking vacuum.
AGILENT 3D CAPILLARY ELECTROPHORESIS SYSTEM
(PART-# G1602A) as per
Consists of the Agilent 3D CE instrument, Agilent Agilent
3D-CE Chem-Station Software, HP Computer, HP country
Laserprinter and OS for Computer price list
AGILENT CE-ESI-MS SPRAYER KIT (PART-# G1607A) as per
Agilent 3D CE-ESI-MS "Triple Layer Needle" Agilent
sprayer kit for coupling of a CE-System to the country
API/ESI Source of the esquire3000 price list
AGILENT 3D CE CE-MS ADAPTER KIT as per
Adapter Kit to connect the Agilent 3D CE System Agilent
to a Mass Spectrometer. Necessary for use of the country
CE with the esquire3000 price list
Exhibit D-8
QTY DESCRIPTION PRICE
------------------------------------------------------------------------------
AGILENT TECHNOLOGIES 1100 HPLC All as per
Agilent
country
The Agilent 1100 HPLC system can be combined to price list
customer's needs from the following set of
subcomponents
o 1100 binary pump G1312A
(Bruker-Part-# 22086)
o 1100 Diode-Array Detector
(Bruker-Part-# 24106)
o (w/o Flow Cell) G1314A
with deuterium lamp
o Standard Flow Cell for AGILENT 1100 DAD OPT021
(Bruker-Part-# 27520)
o 1100 Vacuum Degasser G1322A
(Bruker-Part-# 22088)
o 1100 Automatic Sampler G1313A
(Bruker-Part-# 16839)
o 1100 Thermostatic Column Holder G1316A
(Bruker-Part-# 23471)
o OPTIONS:
o 1100 Quaternary Pump G1354 A
o 1100 Option for G 1312A with sealing rinse for
use with high concentration salt solutions OPT 030
o 1100 Manual Sample Injector G1328A
o 1100 Option for G1316A 4-way column switching
OPT 055
o 1100 DAD with deuterium and tungsten lamp (w/o
Flow cell) G1315A
o 1100 Option 055 for G1315A Flow Cell
o 1100 Control Module G 1323 A
o ChemStation Additional License G2175AA
BRUKER DALTONICS N(2) GENERATOR LC-MS-NGM-11
(PART-#26875) E 6,620.00
The nitrogen generator LC-MS-NGM 11 produces
nitrogen continuously up to a max. purity of 99,5%.
o prefiltration system to remove particles.
o membrane based separation
o no moving parts
o LC-MS-NGM 11 does not need any electrical power
Exhibit D-9
QTY DESCRIPTION PRICE
------------------------------------------------------------------------------
BRUKER DALTONICS N(2) GENERATOR LC-MS-NGM-11 K
(PART-#72461) E 10,000.00
Upon request, the Bruker LC-MS-NGM 11K model with
a built-in low noise and oil-less air compressor
for permanent use (less than 50 dbA) is available
(indicate required voltage)
HP P1100 21" MONITOR (PART-# 30313) E 1,330.00
Trinitron Tube, supports Ultra VGA (1024 X
768)60/75/85Hz, Ultra VGA 1600 (1600 x
1200)60/75/85Hz, Ultra VGA 2 (1280 x 1024)60/75/85
Hz
SOFTWARE OPTIONS
BIOTOOLS (PART-#28693) E 2,950.00
BioTools NT Based Analysis and Interpretation
Package. Supports processed spectra and/or peak
picking results from the esquire3000 ion trap
system, FLEX(TM) III series of MALDI-TOF mass
spectrometers, BioTOF(TM) orthogonal ESI-TOF
system, and APEX(TM) II series of FTMS systems.
o Internet library searches are fully integrated
in the software using EMBL PeptideSearch and
MASCOT (peptide fingerprint and MS/MS) Requires
Windows NT (4.0 with Service pack 4, Microsoft
Internet Explorer 4.0 or greater) PC with Pentium
II CPU, minimum 300 MHz, graphic resolution 1024
* 768 pixel with 256 colors or better, 128
MBytes of RAM or better, 4 GBytes or larger hard
disk (SCSI recommended), 3.5" Floppy Drive with
1.44 MB capacity, CD-ROM Drive (4x or greater),
and Ethernet interface (Computer not included)
GPMAW (32 BIT)(PART-# 21521) E 218.00
The standard Windows NT based protein mass
analysis program by ▇▇▇▇▇ ▇▇▇▇▇▇, Odense
University. Designed for protein digest MS/MS
analysis. Includes local sequence database
searches for low throughput work. Import filters
for esquire3000 peak lists and FLEX. Free
Software upgrade via GPMAW Internet homepage
Exhibit D-10
QTY DESCRIPTION PRICE
---------------------------------------------------------------------------------------------
MASCOT (PART-#28795) E 5,625.00
Intranet version of the Internet search software from Matrix Science.
The software enables OWL, NCBInr and dbEST protein sequence database
searches using MS and MS/MS (PSD) data.
- No interpretation - even partial - needed for MS/MS searches!
- BATCH SEARCHES FROM COMPLETE (ESQUIRE 3000) LC-MS/MS RUNS ARE ALSO
SUPPORTED. AND ARE AN INTEGRAL PART OF THE MS BIOTOOLS SOFTWARE.
REQUIRES AN NT-WORKSTATION WITH SERVER SOFTWARE
BRUKER DALTONICS DATAANALYSIS POST PROCESSING SOFTWARE PACKAGE DA 2.0 E 3,700.00
(PART-#72669)
- Intuitive treeview-TM- for data-handling
- Unified handling of profile spectra and mass spectra
- full 32-bit support
- easy-to-learn
- easy-to-use
BRUKER DALTONICS QUANTANALYSIS-TM- MODULE FOR DA 2.0 E 750.00
BRUKER DALTONICS LIBRARYSEARCH-TM- MODULE FOR DA 2.0 E 700.00
BRUKER DALTONICS CHARGE DECONVOLUTION MODULE FOR DA 2.0 E 400.00
AGILENT DECONVOLUTION/BIOANALYSIS (PEPTIDE TOOLS)(PART-#22190) E 7,250.00
Software package consisting of the Bruker DA deconvolution option
and AGILENT Peptide Tools (G1981AA):
AGILENT PEPTIDE TOOLS SOFTWARE PACKAGE W/0 DECONVOLUTION OPTION E 3,050.00
(PART-#20419)
MISCELLANEOUS
TRAINING COURSE E 1,950.00
Three day extended training course at the factory in Bremen
(Price is per person and excludes travel and lodging, 8 hours training
per day))
Exhibit D-11
GENERAL TERMS AND CONDITIONS
WARRANTY
One (1) year after acceptance, but not longer than 15 months after
delivery. Warranty covers both parts and labor. For items supplied but
not manufactured by Bruker, the warranty terms of the manufacturer will
be transferred to the buyer. The warranty does not cover consumables,
columns, capillaries, needles, routine pump maintenance (e.g. cleaning,
oil change, etc.), digital media, solvents, etc.
TERMS AND CONDITIONS
All prices are quoted F.O.B. Bremen and do not include import duty, and
do not include state, local or any other taxes. Buyer will supply with
the purchase order a certificate that purchase is exempt from sales tax or
that buyer will directly remit sales/use tax, if any.
PAYMENT TERMS
Irrevocable Letter of Credit with:
40% upon order
40% upon delivery*
20% upon demonstration of specification
* If delivery of the system is delayed by request of the purchaser, then
the amount due upon delivery will be considered payable on the quoted
delivery date.
DELIVERY
Approximately 60 days ARO
INSTALLATION
Installation includes familiarization on-site by the installation
engineer. Additional factory training is available as an option. Please
contact Bruker for further details.
SITE PREPARATION
Please see the Site Preparation Document for details
Exhibit D-12
CONSUMABLES PRICE LIST
Please contact Bruker Daltonik GmbH, Bremen, Germany at ▇▇▇-▇▇▇-▇▇▇▇-▇
for information on spare parts.
LIST-PRICE E
CONSUMABLES
MISCELLANEOUS
Filter Nitrogen 21755 225,00
Vials pre-backed sample 1ml (100 pcs) 21756 12,00
Caps snap-on, teflon only (100 pcs) 21757 12,00
Microliter Syringe, 250ul 21232 45,00
Fill Port Assembly, 1/16" 18218 ▇▇,▇▇
▇▇▇ ▇▇▇▇▇, ▇▇, ▇/▇▇", 0,020mm 21737 73,00
Septa Thermogreen LB-2, D=10mm (50 pcs) 47445 79,00
Fitting (10 pcs) 21150 24,00
Union 21151 38,00
Tubing, PEEK-; 1/16", 1,5m 80172 18,00
Rough Pump Oil, Inland 45 20221 -
Rough Pump Oil, Ultragrade 19 (Can with 4liters) 49777 37,00
Pipette, 0.5ul - 5ul (200 pieces in a pack) 47008 43,00
CHEMICALS AND CLEANING TOOLS
Exhibit D-13
Reserpine 5ng/ul (AGILENT Part-#G2423A) 21792 136,00
Calibration Tune Mix standard mass range 18219(100ml) 171,00
(AGILENT Part-#G2431A)
Calibration Tune Mix extended mass range 18220(100ml) 171,00
AGILENT Part-#G2421A)
Calibration Tune Mix APCI (AGILENT Part-#G2422A) 27227 -
Acetonitril Chromasolv (1000 ml) 44108 30,00
Water for Chromatography (1000 ml) 42111 9,00
Exhibit D-14
DESCRIPTION LIST-PRICE E
CHEMICALS AND CLEANING TOOLS
2-Propanol Chromasolv (1000 ml) 21816 19,00
Cloths, clean, lint free, Kimwipes (1 box) 68343 3,00
Gloves, clean, lint free, Size 9 1/2 (1 box) 01213 6,00
Exhibit D-15
FLEX
SERIES
FULLY AUTOMATED MALDI-TOF MASS SPECTROMETERS
FOR
ROUTINE HIGH-PERFORMANCE HIGH-THROUGHPUT APPLICATIONS
[PHOTO DESCRIPTION TO COME]
IN
PROTEOMICS, GENOMICS, PHARMACOGENOMICS, PROTEIN
ANALYSIS, PEPTIDE & OLIGONUCLEOTIDE SEQUENCING, AND
SYNTHETIC POLYMERS
Exhibit D-16
DESCRIPTION AND EUROPEAN PRICE LIST
Exhibit D-17
TABLE OF CONTENTS
3 INTRODUCTION
8 COMPLETE MALDI-TOF SYSTEMS, OPTIONS, AND PRICING
17 COMPUTER & SOFTWARE OPTIONS
20 SCOUT-TM- MTP AND SCOUT-TM- 100 MALDI TARGETS
23 TRAINING, SERVICE, AND MAINTENANCE
25 GENERAL TERMS & CONDITIONS
Exhibit D-18
FLEX
SERIES
INTRODUCTION
Exhibit D-19
THE FLEX MALDI-TOF MASS SPECTROMETER
PRODUCT LINE
The FLEX-TM- series of MALDI-TOF mass spectrometers offers a full range of
performance from the REFLEX-TM- III with the ultimate performance and
flexibility, to the research grade high-throughput BIFLEX III and finishing
with high performance and easy to use walk up bench-top OmniFLEX. Common to
the entire line is intelligent automation with "fuzzy logic" based
AutoXecute-TM- for problem solving in the analytical and bio-analytical
lab-oratories with a wide variety of applications. Examples in Proteomics,
Genomics, Pharmacogenomics, protein analysis, peptide and oligonucleotide
sequencing, and synthetic polymer analysis are well documented in product
notes, application notes and reprints of scientific literature available from
Bruker Daltonics. All FLEX MALDI-TOF mass spectrometers offer the highest
performance available in their price class.
GRIDLESS ION SOURCES AND REFLECTRONS
Bruker Daltonics pioneered the first commercial gridless two stage reflectron
based TOF mass spectrometer almost two decades ago and was the first to apply
MS/MS to MALDI-TOF MS for peptides with FAST (Fragmentation Analysis and
Structural TOF) for PSD (Post Source Decay) measurements of metastable ions.
With the introduction of PIE (pulsed ion extraction) Bruker added ISD
(In-Source Decay) for sequencing even on "linear only" instruments. The
gridless design of both the ion sources and reflectrons of the FLEX series of
MALDI-TOF mass spectrometers eliminates the ion loss and scattering that
occurs
Exhibit D-20
with lower performance gridded systems to give both high mass accuracy and
the highest sensitivity possible especially at higher molecular weights.
SCOUT-TM- MTP AND SCOUT-TM- 100 SAMPLE INLETS
BRUKER DALTONICS' REFLEX-TM- III AND BIFLEX-TM- III MALDI-TOF MS SYSTEMS
OFFER THE INNOVATIVE SCOUT-TM- MTP AUTOMATED SAMPLE TARGET IN MICROTITRE
FORMAT AS THE STANDARD INLET SYSTEM. WITH X150 MAGNIFICATION OPTICS, THE
SCOUT MTP ALLOWS FOR DETAILED EXAMINATION OF THE SAMPLE SPOT. FOR HIGH
THROUGHPUT, AUTOMATED MALDI-TOF WITH UNATTENDED ACQUISITION, THE COMBINATION
OF THE SCOUT MTP MICROTITRE FORMAT WITH THE POWERFUL AUTOXECUTE-TM- SOFTWARE
AND PRECISE X-Y POSITIONING WITH 4 MICRONS STEP-SIZE OFFERS UNRIVALLED
REPRODUCIBILITY BETWEEN SAMPLE SPOTS. "FUZZY LOGIC" ALGORITHIMS OPTIMIZE FOR
HIGHEST QUALITY DATA FOR UP TO 1,536 SAMPLES ON 1 TARGET. THE SCOUT MTP
OFFERS BOTH THE LARGEST PHYSICAL TARGET AND THE LARGEST CAPAC-ITY IN THE
INDUSTRY WHILE LEVERAGING EXISTING AUTOSAMPLER/ROBOT TECHNOLOGY BY USING AN
IDENTICAL PATTERN FORMAT AND PHYSICAL DIMENSIONS AS A MICROTITRE PLATE. THE
SCOUT MTP WITH THE MAP II OR MAPII/8 SAMPLE ROBOT (SEE BELOW) ARE IDEAL FOR
HIGH VOLUME BATCH ANALYSIS IN LARGE INDUSTRIAL PRODUCT DEVELOPMENT PROGRAMS.
In multi-user environments where several groups with different sample classes
need a cost-effective easy to use bench-top system, the OmniFLEX-TM- with the
SCOUT-TM- 100 inlet system is ideal as a walk up instrument. Based on the
rugged and proven SCOUT 26 sample inlet, the SCOUT 100 offers different
targets with up to 100 positions. When combined with AutoXecute (see below)
the OmniFLEX with the SCOUT 100 is the first bench-top MALDI-TOF MS system
worth buying to achieve high quality data from an economical instrument.
Exhibit D-21
AUTOMATION
The demand for high throughput automated sample preparation and analysis for
a wide range of sample classes is rapidly increasing. This is addressed by
Bruker with the SCOUT MTP, AutoXecute, and with the Bruker Daltonics MAP II
and MAPII/8 (MALDI Auto Preparation) sample robots based on the popular
▇▇▇▇▇▇ 215 and ▇▇▇▇▇▇ Multiprobe robot, resp.. The robots of the MAP II
series MAP II have racks that can each hold up to ten microtitre plates, as
well as a variety of other sample formats including a wide variety of
standard glass and plastic sample vials. This allows the Bruker user to
prepare automatically a large number of samples easily and reproducibly in a
batch mode directly on the MALDI target.
For automated data acquisition, the AutoXecute acquisition interface
includes "fuzzy logic" algorithms for fast automated optimization of the
instrument for almost any sample class without operator intervention. This
includes automatic optimization of proteins, peptides, oligonucleotides,
carbohydrates and synthetic polymers samples with automatic processing,
plotting and report generation. Using these automation features, it is not
even necessary for the instrument operator to view the spectrum for many
routine samples.
SOFTWARE AND COMPUTER OPTIONS
The Bruker Daltonics FLEX product line comes standard with the XACQ data
acquisition including AutoXecute and the XMASS-TM- data processing package,
running on industry standard workstations. For off-line processing and data
inspection, XMASS is available in a processing-only
Exhibit D-22
version (no spectrometer control or data acquisition capabilities) for
Windows NT computer platforms.
As the first Bruker Daltonics MALDI-TOF MS the OmniFLEX is fully operated on
a Windows NT computer platform.
The Bruker NT-based software package, MS BioTools-TM-, supports data analysis
and interpretation of data generated from all Bruker Daltonics mass
spectrometers used in life science systems or as analytical instruments. MS
BioTools provides communication and file import/export for XMASS single
spectra, FAST-TM- (post-source decay) spectra, LC/MS ion trap profile
spectra, deconvoluted single charged profile spectra, GPMAW sequence files
(unmodified amino acids only) and clipboard support for spectra and data.
Processing functions include annotation of spectra using a given amino acid
sequence, matching of experimental spectra with potential amino acid sequence
candidate lists. In addition, MS BioTools interprets spectra and automatically
generates sequence tags for Internet database searches using EMBL
PeptideSearch. Other search engines include the MASCOT package which allows
to search using not interpreted MS/MS spectra.
Exhibit D-23
FLEX
SERIES
COMPLETE MALDI-TOF SYSTEMS, OPTIONS, AND PRICING
EXHIBIT D-24
REFLEX-TM- III - THE ULTIMATE-PERFORMANCE MALDI TOF MS SYSTEM
1. TOF-100: REFLEX-TM- III MALDI TOF MS SYSTEM
TIME-OF-FLIGHT MASS ANALYZER
- 150 cm linear TOF analyzer for both positive and negative ions
- Fast, high-sensitivity and high dynamic range gated MCP ion detector
system
- Ion flight path housing and vacuum system stand
- Ultrastable power supplies for TOF analyzer, detector and ion source
- Includes +25/-20 kV HV power supplies for ion acceleration
GRIDLESS MALDI SOURCE WITH PULSED ION EXTRACTION (PIE-TM-)
- Modular ion source housing
- Valve between ion source and TOF analyzer
- Ion lens system with matrix ion elimination system
- Automatic vacuum lock for sample introduction with inlet vacuum pump
- N2-LASER including variable power attenuator and UV optics
- SCOUT-TM- MTP Microtitre Plate Source / Target with Observation Optics
- Large area target with exact dimensions of microtitre plate
- Precise X-Y positioning (4 micron step increments)
- 2 exchangeable targets (384-position standard)
- 1 adapter target for up to 12 inserts provided with 25 assorted
inserts (10 position)
- High resolution magnifying observation optics with display on 14"
color monitor
- Intuitive GUI for simple mouse controlled X-Y positioning
PUMPING SYSTEM INCLUDING VACUUM MEASUREMENT AND CONTROL UNIT
- 260 I/sec turbomolecular pump including fore-pump for the mass analyzer
- 260 I/sec turbomolecular pump including fore-pump for the ion source
DATA SYSTEM AND SOFTWARE
- 2 GHz Digitizer standard
- Sun SPARCUltra 5, 19" color monitor, 128 MB RAM, 4.3 GByte IDE disk,
1.44 MB floppy drive, CD-ROM drive, Ethernet connection for external
networks
- Mass spectrometry software for acquisition (XACQ-TM-), processing
(XMASS-TM-), plotting, and analysis in a networked multi-user
environment
- AutoXecute-TM- with fuzzy-logic optimization for automated acquisition
- Postscript printer
- DAT tape drive for backup
Exhibit D-25
SYSTEM LIST PRICE EURO 226,000.00
Exhibit D-26
REFLEX-TM- III OPTIONS
MASS ANALYZER OPTIONS
2. TOF-105 ADDITION OF GRIDLESS ELECTROSTATIC ION REFLECTOR EURO 52,200.00
- Patented gridless two-stage ion reflectron for increased
mass resolution and accuracy with PIE-TM- operation
- Includes ion reflectron electronics
- Includes special second high sensitivity fast 5 micron
MCP detector and power supplies
3. TOF-110 FAST (FRAGMENTATION ANALYSIS AND STRUCTURAL TOF-MS) EURO 17,400.00
- Accessory for PSD (Post-Source Decay) Experiments
- Independent reflector power supply with high-precision DACs
- FAST software for calibration, pasting of segments, etc.
- FAST-FILTER-TM- high-resolution parent ion preselector for
true MS/MS
- Reflector and 2nd detector (TOF-105) required
4. TOF-115 CID (COLLISION-INDUCED DISSOCIATION) ACCESSORY EURO 8,700.00
- Includes gas inlet valve/CID interface in source
- Includes control software (operates with and requires
FAST Accessory (TOF-110)
5. TOF-130 SUBSTITUTE HIMAS-TM- DETECTOR IN LIEU OF LINEAR EURO 10,440.00
MCP DETECTOR
- ULTIMATE GAIN DETECTOR FOR HIGH M/Z SYNTHETIC POLYMER
APPLICATIONS
- Reduced time resolution at very low m/z
- Very wide dynamic range for maximum ion handling capacity
LASER OPTIONS
6. TOF-150 ND:YAG LASER (IN ADDITION TO N2-LASER) EURO 33,000.00
- Includes crystals for both 355 nm and 266 nm operation
7. TOF-155 IR LASER (IN ADDITION TO N2-LASER) EURO 65,250.00
- Includes set up for IR MALDI or UV MALDI
- Includes all appropriate IR optics
- IR Laser is an Erbium-YAG with 2.94 micron wavelength
Exhibit D-27
AUTOMATED SAMPLE PREPARATION OPTIONS
8. TOF-170 MAP II (MALDI Auto Preparation) Sample and EURO 20,800.00
Liquid Handler
- Based on ▇▇▇▇▇▇ 215 autosampler with liquid handler and
injector, customized for MALDI application with disposable
tips
- Racks available for a wide variety of sample vials, and 96
and 384 well plates
- Fully computer controlled from Windows NT data station under
MS Excel (not included), includes all control software and
all user-interface software with Bruker MALDI applications
software
9. TOF-171 MAP II/8 Sample and Liquid Handler EURO 45,950.00
- Based on ▇▇▇▇▇▇ Multiple Probe 215 autosampler, 8-channel
liquid handler and injector, customized for MALDI application
with disposable tips
- Racks available for a wide variety of sample vials, and 96
and 384 well plates
- Fully computer controlled from Windows NT data station under
MS Excel (not included), includes all control software and all
user-interface software with Bruker MALDI applications software
- Higher performance than MAP II due to 8 channel operation
10. TOF-175 HOOD FOR MAP II ROBOT EURO 4,590.00
SOFTWARE AND COMPUTER OPTIONS (PLEASE REFER TO PAGE 17)
Exhibit D-28
BIFLEX (TM) III RESEARCH-GRADE MALDI-TOF MS SYSTEM
11. TOF-200: BIFLEX (TM) III MALDI-TOF MS SYSTEM
TIME-OF-FLIGHT MASS ANALYZER
- 125 cm linear TOF analyzer for both positive and negative ions
- High sensitivity fast MCP detector system with detector housing
- Ion flight path housing
- Integrated vacuum system, electronics and computer desk
- Ultra-Stable power supplies for TOF analyzer, detector and ion source
GRIDLESS MALDI SOURCE WITH PULSED ION EXTRACTION (PIE (TM))
- Ion source and ion lens system
- Automatic vacuum lock for sample introduction with inlet vacuum pump
- N2-LASER including variable power attenuator and UV optics
- SCOUT (TM) MTP Microtitre Plate Source/Target with Observation Optics
- Large area target (12cm x 8cm) with exact dimensions of microtitre
plate
- Precise X-Y positioning (4 micron step increments)
- 2 exchangeable targets (384-position standard)
- 1 adapter target for up to 12 inserts provided with 25 assorted
inserts (10 position)
- High resolution magnifying observation optics with display on 14"
color monitor
- Intuitive GUI for simple mouse controlled X-Y positioning
PUMPING SYSTEM INCLUDING VACUUM MEASUREMENT AND CONTROL UNIT
- 260 I/sec turbomolecular pump including fore-pump
- Vacuum measurement system and control unit
DATA SYSTEM AND SOFTWARE
- 2 GHz Digitizer
- Sun SPARCUltra 5, 19" color monitor, 128MB RAM, 4.3 GByte IDE disk,
1.44 MB floppy drive, CD-ROM drive, Ethernet connection for external
networks
- Mass spectrometry software for acquisition (XACQ (TM)), processing
(XMASS (TM)), plotting, and analysis in a networked multi-user
environment
- AutoXecute with fuzzy-logic optimization for automated acquisition
- Postscript printer
- DAT tape drive for backup
System List Price E 172,250.00
Exhibit D-29
BIFLEX (TM) III OPTIONS
MASS ANALYZER OPTIONS
12. TOF-205 ADDITION OF GRIDLESS ELECTROSTATIC ION REFLECTOR FOR E 43,500.00
BIFLEX (TM) III
- Gridless ion reflectron for increased mass resolution and
accuracy with PIE (TM) operation
- Includes ion reflectron electronics
- Includes second high sensitivity fast MCP detector and
power supplies
13. TOF-210 FAST (FRAGMENTATION ANALYSIS AND STRUCTURAL TOF-MS) E 17,400.00
- Accessory for PSD (Post-Source Decay) Experiments
- Independent reflector power supply with high-precision
DACs
- FAST software for calibration, pasting of segments, etc.
- FAST-FILTER (TM) high-resolution parent ion preselector
for true MS/MS
- Reflector and 2nd detector (TOF-205) required
14. TOF-215 CID (COLLISION-INDUCED DISSOCIATION) ACCESSORY E 8,700.00
- Includes gas inlet valve/CID interface in source
- Includes control software (operates with and requires
FAST Accessory (TOF-210)
15. TOF-230 SUBSTITUTE HIMAS (TM) DETECTOR IN LIEU OF LINEAR E 10,440.00
MCP DETECTOR
- Ultimate gain detector for high m/z synthetic polymer
applications
- Reduced time resolution at very low m/z
- Very wide dynamic range for maximum ion handling capacity
Exhibit D-30
AUTOMATED SAMPLE PREPARATION OPTIONS
16. TOF-170 MAP II (MALDI Auto Preparation) Sample and Liquid E 20,800.00
Handler
- Based on ▇▇▇▇▇▇ 215 autosampler with liquid handler and
injector, customized for MALDI application with
disposable tips
- Racks available for a wide variety of sample vials, and
96 and 384 well plates
- Fully computer controlled from Windows NT data station
under MS Excel (not included), includes all control
software and all user-interface software with Bruker MALDI
applications software
17. TOF-171 MAP II/8 Sample and Liquid Handler E 45,950.00
- Based on ▇▇▇▇▇▇ Multiple Probe 215 autosampler,
8-channel liquid handler and injector, customized for
MALDI application with disposable tips
- Racks available for a wide variety of sample vials, and
96 and 384 well plates
- Fully computer controlled from Windows NT data station
under MS Excel (not included), includes all control
software and all user-interface software with Bruker
MALDI applications software
- Higher performance than MAP II due to 8 channel operation
18. TOF-175 HOOD FOR MAP II ROBOT E 4,590.00
SOFTWARE AND COMPUTER OPTIONS (PLEASE REFER TO PAGE 17)
Exhibit D-31
OmniFLEX(TM) BENCH-TOP MALDI TOF MS
19. ▇▇▇ - ▇▇▇ ▇▇▇▇▇▇▇▇(▇▇) ▇▇▇▇▇-▇▇▇ ▇▇▇▇▇ TOF MS
LINEAR TIME-OF-FLIGHT MASS ANALYZER
- 120 cm linear TOF analyzer
- Positive and negative ion detection
- High sensitivity fast MCP detector system with detector housing
- Integrated vacuum system and electronics with compact Bench-Top
footprint
GRIDLESS MALDI SOURCE WITH PULSED ION EXTRACTION (PIE(TM))
- SCOUT(TM) 100 X-Y stage ion source with 100-position target and
magnifying observation optics
- Gridless design for maximum sensitivity
- Precise X-Y positioning of sample allowing access to all target
area
- Easy to use GUI for simple mouse control of system
- Automatic vacuum lock for sample target introduction
- Computer-controlled UV LASER (N(2) @ 337nm)
- 4 exchangeable targets (2 x 100 position, 2 x 49 position)
PUMPING SYSTEM INCLUDING VACUUM MEASUREMENT AND CONTROL UNIT
- 70 I/sec turbomolecular pump including fore-pump
- Vacuum measurement system and control unit
DATA SYSTEM AND SOFTWARE
- 1 GHz 8-bit Digitizer
- Windows-NT workstation: 19" color monitor, 256MB RAM, >8 GByte
hard disk, 1.44 MByte floppy drive, CDR drive for data archival,
Ethernet connection for external networks
- Video camera capture card for on-screen display of target spot
- Mass spectrometry software for data acquisition (NT-Acquisition),
processing (XMASS-NT(TM)), plotting, and analysis in a networked
multi-user environment
- Laser printer for data output
SYSTEM LIST PRICE E 130,000.00
Exhibit D-32
OmniFLEX(TM) OPTIONS
20. TOF - 505 HIGH RESOLUTION /PSD OPTION FOR OmniFLEX(TM) E 50,000.00
- Addition of Gridless Electrostatic Ion Reflectron and control
electronics
- Includes second detector with high speed dual MCP technology
- FAST(TM) Post Source Decay accessory with pre-cursor ion
selection device for structural analysis. Automatic PSD
pasting/calibration capability
- Includes all relevant control software, hardware and power
supplies
SOFTWARE AND COMPUTER OPTIONS (PLEASE REFER TO PAGE 17)
Exhibit D-33
FLEX
SERIES
COMPUTER & SOFTWARE OPTIONS
Exhibit D-34
COMPUTER & SOFTWARE OPTIONS
COMPUTER OPTIONS
21. TOF-400 SUBSTITUTE 19" COLOR MONITOR FOR 17" COLOR MONITOR E 1,700.00
22. TOF-410 SUBSTITUTE MO DRIVE IN LIEU OF DAT TAPE FOR BACKUP E 1,000.00
- For very fast data access
23. TOF-415 HEWLETT PACKARD COLOR LASER PRINTER E 5,250.00
- HP Laser Jet 4200 DN Color Postscript laser printer
SOFTWARE OPTIONS
24. TOF-440 ADDITIONAL LICENSE FOR XMASS, UNIX VERSION E 3,100.00
- License to run XMASS on one additional SGI or SUN UNIX workstation,
for offline data processing, does not include installation.
25. TOF-445 ADDITIONAL LICENSE FOR XMASS, NT VERSION E 3,100.00
- License to run XMASS on one additional NT workstation, for offline
data processing, does not include installation.
26. TOF-450 MS BIOTOOLS NT BASED ANALYSIS AND INTERPRETATION PACKAGE E 2,950.00
- Supports processed spectra and/or peak picking results from
FLEX(TM)III series of MALDI-TOF mass spectrometers, BioTOF(TM)
orthogonal ESI-TOF system, ESQUIRE(TM)-LC ESI ion trap system, and
APEX(TM)II series of FTMS systems.
- Communication and File Import/Export of XMASS spectra, FAST(TM)
(post source decay) spectra, LC ion trap profile spectra from
Bruker/HP ESQUIRE-LC systems, deconvoluted single charged profile
spectra, GPMAW Sequence Files (unmodified amino acids only), and
clipboard support for spectra and data
- Annotation of spectra and ▇▇▇▇▇▇▇▇ ▇▇▇▇ to visualize and score the
match between MS/MS spectra and the sequence.
- Automatic generation of sequence tags for database searches
- Internet library searches are fully integrated in the software using
EMBL PeptideSearch and MASCOT (peptide fingerprint and MS/MS)
- Requires Windows NT (4.0 with Service pack 3, Microsoft Internet
Explorer 4.0 or greater) PC with Pentium II CPU, minimum 300 MHz,
graphic resolution 1024 * 768 pixel with 256 colors or better, 128
MBytes of RAM or better, 4 GBytes or larger hard disk (SCSI
recommended), 3.5" Floppy Drive with 1.44 MB capacity, CD-ROM Drive
(4x or greater), and Ethernet interface (Computer not included)
Exhibit D-35
SOFTWARE OPTIONS (CONT.)
27. TOF-455 GPMAW E 216.00
The Standard in Protein MS, NT based Version Protein mass analysis
program from ▇▇▇▇▇ ▇▇▇▇▇▇, Odense University.
Designed for protein digest MS/MS analysis. Includes local sequence
database searches for low throughput work. Import filters for FLEX and
ESQUIRE(TM) LC peak lists.
Software upgrade free via GPMAW Internet homepage.
28. TOF-460 MASCOT E 5,625.00
Intranet version of the Internet search software. From Matrix Science.
The software enables OWL, NCBlnr and dbEST protein sequence database
searches using MS and MS/MS searches! Also batch searches from complete
LC-MS/MS runs (ESQUIRE) are supported. Searches are supported under
AutoXecute and XMASS and are an integral part of the MS BioTools
software. (e.g., Personal Web Server from Microsoft, free).
Single Processor License.
29. TOF-461 MASCOT E 11,250.00
Double Processor License.
30. TOF-462 MASCOT E 22,500.00
Quadruple Processor License.
Exhibit D-36
FLEX SERIES
SCOUT-TM- MTP AND SCOUT-TM- 100 MALDI TARGETS
Exhibit D-37
SCOUT-TM- MTP MALDI TARGETS
ONE-PIECE ALUMINUM TARGETS
31. #26755 384 TARGET E 254.00
- With anti-corrosive coating, ground
32. #26993 384 TARGET E 283.00
- With Gold coating, ground
ANCHORCHIP-TM- TARGET PLATES
To be mounted on Frame (#24983) with Key (#26475)
33. #72226 ANCHORCHIP-TM- 200/384 (384 ANCHORS, 200-mu-m DIAMETER) E 414.00
34. #72227 ANCHORCHIP-TM- 400/384 (384 ANCHORS, 400-mu-m DIAMETER) E 414.00
35. #72228 ANCHORCHIP-TM- 600/384 (384 ANCHORS, 600-mu-m DIAMETER)
36. #72229 ANCHORCHIP-TM- 800/384 (384 ANCHORS, 800-mu-m DIAMETER)
37. #29414 ANCHORCHIP-TM- VAR/384 E 414.00
- 384 Anchors, four different Anchor Diameter: 96x200-mu-m, 96x400-mu-m,
96x600-mu-m, 96x800-mu-m
38. #72448 ANCHORCHIP-TM- 200/1536 (1536 ANCHORS, 200-mu-m DIAMETER) E 470.00
39. #72449 ANCHORCHIP-TM- 400/1536 (1536 ANCHORS, 400-mu-m DIAMETER) E 470.00
SANDWICH TARGETS AND ACCESSORIES
40. #25183 384 TARGET PLATE, STAINLESS STEEL, GROUND E 103.00
41. #25185 864 TARGET PLATE, STAINLESS STEEL, GROUND E 157.00
42. #25184 384 TARGET PLATE, STAINLESS STEEL, POLISHED E 157.00
43. #26983 384 TARGET PLATE, GLASS, GROUND E 377.00
- With electrical conductivity coating, ultra flat
Exhibit D-38
Exhibit D-39
SCOUT-TM- MTP MALDI TARGETS (Cont.)
44. #▇▇▇▇▇ ▇▇▇▇▇ ▇▇▇ TARGET FRAME E 377.00
45. #▇▇▇▇▇ ▇▇▇ ▇ 62.00
- For sandwich target assembly
SPECIAL TARGETS
46. #24996 MULTIPROBE ADAPTER TARGET E 560.00
47. #26762 PEBio ADAPTER TARGET E 412.00
SCOUT-TM- MALDI TARGETS
48. 100-POSITION TARGET (REF: A3315) E 200.00
49. 49-POSITION TARGET (REF: A3316) E 200.00
Exhibit D-40
FLEX SERIES
TRAINING, SERVICE, AND MAINTENANCE
Exhibit D-41
TRAINING, SERVICE, AND MAINTENANCE
50. FACTORY TRAINING COURSE E 1,950.00
- 3 day course at the factory
- Includes instrument control, data acquisition and processing
- Includes sample preparation and application-specific training
- Price is per person and excludes all travel & lodging expenses
51. SERVICE & MAINTENANCE CONTRACTS E variable
are available on request. Please ask at your local Bruker Daltonics
office.
Exhibit D-42
FLEX SERIES
GENERAL TERMS & CONDITIONS
Exhibit D-43
GENERAL TERMS AND CONDITIONS
WARRANTY TERMS
One (1) year after acceptance, but not longer than 15 months after delivery.
Warranty covers both parts and labor. For items supplied but not manufactured
by Bruker, the warranty terms of the manufacturer will be transferred to the
buyer. The warranty does not cover consumables, columns, capillaries,
needles, routine pump maintenance (e.g. cleaning, oil change, etc.), digital
media, solvents, etc.
TERMS AND CONDITIONS
All prices are quoted F.O.B. Bremen, Germany, and do not include import duty,
and do not include state, local or any other taxes. Buyer will supply with
the purchase order a certificate that purchase is exempt from sales tax or
that buyer will directly remit sales/use tax, if any.
PAYMENT TERMS
Irrevocable Letter of Credit with:
40% upon order
40% upon delivery*
20% upon demonstration of specification
Exhibit D-44
*If delivery of the system is delayed by request of the purchaser, then the
amount due upon delivery will be considered payable on the quoted delivery
date.
Exhibit D-45
DELIVERY
Approximately 60, max. 90 days ARO
INSTALLATION
Installation includes familiarization on-site by the installation engineer.
Additional factory training is available as an option. Please contact Bruker
for further details.
SITE PREPARATION
Site Preparation Document for details will be disseminated timely enough by
your local Bruker Daltonics office.
Exhibit D-46
[GRAPHIC DESCRIPTION TO COME]
INTRODUCTION
Dear Customer,
as you will certainly understand, it is impossible for us to visit future
installation sites in order to make sure that everything is perfectly
prepared for the installation of your new instrument. So we have to put this
task into our customer's hands.
However, with this document in hand and some telephone numbers as a backup,
the site preparation for your new esquire3000(TM) should not be a problem.
Please follow the directions given in this manual and make sure, that the
installation site complies with your local laws, regulations, codes and
ordinances with regard to electrical and mechanical installations, building
safety and use of hazardous materials/chemicals.
In order to schedule the installation as flexible as possible, please use the
enclosed form in the appendix of this document for your fax reply to the
BRUKER Daltonics Service Team. This fax does also confirm the preparation of
the installation site.
Note, that it is not possible for us to schedule your installation prior to
the receipt of your reply fax.
If there are any questions related to the site preparation for your new
esquire3000(TM), please do not hesitate to contact us by phone or e-mail.
In appreciation of your cooperation,
Your BRUKER Daltonics Service Team
Page 1 of 8
[GRAPHIC] Read these instructions below before all continuos actions:
PURPOSE OF PROCEDURE
To ensure that the installation site is properly evaluated and prepared with
the appropriate utilities, consumables and supplies for the successful
installation of the esquire3000(TM) system.
CUSTOMER RESPONSIBILITIES
Customers should ensure those all-necessary operating supplies, consumables
and usage dependent items such as columns, vials, syringes and solvents
required for the successful installation of instruments and systems are
available.
Installation sites should be prepared in accordance with the following
specifications.
IMPORTANT INFORMATION
If you have problems in providing any of the following, please contact your
local BRUKER office for assistance.
Assistance with user specific applications may be provided but should be
contracted separately. Users of the instrument should be present throughout
the installation and familiarization otherwise important operational,
maintenance and safety information may be missed.
RESPONSIBILITIES OF THE LOCAL BRUKER OFFICE
The local BRUKER office will contact you to determine the required power cord
and national plug appropriate for your country and site. Please look up the
appendix for more details.
PROCEDURE CHECKLIST
RECEIVING THE INSTRUMENT /X/
Examine the shipping container for any obvious external damage after the
receipt. In the case of an visible damage, make a note on the freight ▇▇▇▇:
Visible damage - Subject to inspection and test.
Do not open the shipping container unless a BRUKER representative is present
- opening of the container without authorized persons will void the receiving
warranty of the instrument:
As part of the complete installation our service engineers will unpack the
instrument and set it up in customers laboratory. Up to begin of the
installation the shipping container should be stored in a closed area - do
not leave them in the open air.
Make yourself sure about the accessibility of the appropriate areas: All
doors, staircases, floors and elevators must be suitable to the weight and
seize of the shipping container. After installation of the instrument the
shipping container pass into customer hands and should not be returned.
[GRAPHIC] ESQUIRE3000(TM) MAINFRAME /X/
LOCATING THE INSTRUMENT
Dimensions, Weight and Space
WEIGHT: 84 kg/185 lb (without packing)
108 kg/238 lb (with packing)
LENGTH: 754 mm
29.68 in
WIDTH: 696 mm
27.4 in
HEIGHT: 552 mm
21.7 in
PACKAGING: 840 mm x 840 mm x 760 mm
33.1 in x 33.1 in x 29.9 in
The installation site for the esquire3000(TM) must provide sufficient bench
space for the optional AGLILENT HPLC (1100 series) or other HPLC systems,
Personal Computer, monitor, printer and other accessories.
Benches must be sturdy enough to support the weight of all considered desk
equipment.
In addition, there must sufficient space around the system for ventilation
and
Page 2 of 8
maintenance access - at least 300 mm (12 in) to the left and front side and at
least 50 mm (12 in) behind the esquire3000(TM) must kept clear.
Data system size and weight depends on the components included in the data
system. Reserve at least 1000 mm (39in) of bench space for the data system.
Typical weights are 34 kg (75 lb) for the data system and 100 kg (220 lb) for
the AGILENT LC.
The rough pump as stand alone equipment can be located on the bench, better
on the floor to have a vibration decoupling. The pump must be close enough to
the esquire3000(TM), because they are connected by a stiff hose. THe available
length of the tube is dependent on the orientation of the instrument - the
maximally length is 1.5 m.
The esquire3000(TM) must also be connected to the drain bottle, which must be
attached underneath the mass spectrometer.
[GRAPHIC] ENVIRONMENTAL CONDITION REQUIREMENTS /X/
Environmental conditioning considerations include temperature, humidity,
airborne dust and exhaust venting.
The esquire3000(TM) is specified for operation under following conditions:
- Operating Temperature: 13 to 35DEG.C (55 to 95DEG.F)
- Operating Humidity: 15% to 95% non-condensing
@ 35DEG.C
Note that the guaranteed analytical specifications will be met only within
the temperature range of 21DEG. C +/- 3DEG.C (70DEG.F +/- 6DEG.F). Regardless
the existing room temperature the temperature variations shall be < 3DEG.C/hr.
[GRAPHIC] The esquire3000(TM) dissipates up to 2000 ▇▇▇▇▇ (6800 BTU/hr).
The data system and additional equipment also contribute
significantly to the cooling load although the exact amount
depend on the configuration.
[GRAPHIC] ELECTRICAL AND POWER SPECIFICATION /X/
The customer is responsible for providing appropriate electrical power and
power outlets for all system components.
The esquire3000(TM) includes a wide-range input that can operate between 208 to
240 VAC +/- 10%, 50/60 Hz +/- 3% without any configuration.
The terminal connection of the power socket is customers responsibility: The
esquire3000(TM) can be powered either with dual phase voltage (208 VAC in
North America e.g.) or single phase voltage (230 VAC in Europe e.g.). The
current cut-out device should not have a protection less than 10 ampere.
Note that the correct grounding of the electrical installation must be
guaranteed by the customer:
It is inadmissible to use the neutral wire as safety ground. The ground wire
should be an isolated ground, carrying zero current except in the case of a
fault. Interruption of the protective conductor can cause a shock hazard for
the user and can damage the instrument.
Page 3 of 8
The single rough pump draws its power from the esquire3000-TM-. However a
different pump version is delivered depending on the mains voltage in your
country, which affects on the efficiency of the pump.
The other low power equipment like the data system also includes a full-range
power supply, or a voltage selector in few cases. Please refer to the
corresponding instruction manuals.
ADDITIONAL CONSIDERATIONS
o Plan extra power capacity for additional equipment.
o Every equipment, like the esquire3000-TM- and the data system,
should have an own separate circuit with a particular breaker.
o If your mains voltage is unstable use an uninterruptable power supply
(UPS).
o Electromagnetic interferences, like NMRs, celluar phones and
radio transmitters may have a bad effect on system performance.
o It is recommended to install an emergency stop to interrupt the
electric circuits and other systems in a emergency case or for
maintenance.
LABORATORY GAS SUPPLY REQUIREMENTS /X/
NITROGEN GAS REQUIREMENTS
The specified performance of the esquire3000-TM- contingent on a constant,
large supply of nitrogen for drying and nebulizing gas.
The nitrogen consumption come to 5 to 15 sl/min in operation (typical
8 to 10 sl/min) and 3 to 4 sl/min in standby. Due to this large quantity of
nitrogen required we recommend following easy ways out:
o NITROGEN GENERATOR, which works as gas separator. The only
requirement is a clean compressed air source with a operating range of
80 to 100 PSI (5 to 7 bar) and a flow rate of at least 10 l/min @ 6 bar.
No additional mains voltage connections are necessary for this
solution.
o LIQUID NITROGEN ▇▇▇▇▇ FLASK (LESS THAN 160 l), which generates a
pressure of 80 to 100 PSI (5 to 7 bar).
o Multiple high pressure NITROGEN CYLINDERS configuration, Quality 5.0
(99.999 % purity), with a minimum demand of 2 cylinders with
coupled outputs. Single nitrogen cylinders are NOT recommended.
NITROGEN PURITY
The nitrogen gas must be free of contaminants in dependence on the source of
the nitrogen. Reason for this are the different types of contaminants usual to
the different nitrogen sources as listed above:
Bottled nitrogen for example have a tendency to be contaminated with
hydrocarbons, which can be the reason for a widely deviation to BRUKER's
specified performance.
Note, if the purity of the bottling is less than 99.999 % even an optional
available gas purifier can be unable to remove all of the hydrocarbon
contaminants.
On the other hand is oxygen the main contaminant which appears with the
Page 4 of 8
use of a nitrogen generator. Oxygen has less influence on the performance
degree, in lower concentrations even a supportive role. Therefore the
purity specification for nitrogen separated by generators can be less than
the one for bottled nitrogen.
HELIUM GAS REQUIREMENTS
To ensure the best system's performance, please follow these characteristics:
o Helium purity with an Ultra High Percentage (UHP) grade of
99.996 % minimum (Quality 4.6).
o Regulator capable of delivering helium in a range of 50 PSI (3.5 bar)
to 150 PSI (10.3 bar) maximum. We recommend a helium pressure
of 65 PSI (4.5 bar) to 80 PSI (5.6 bar) for the instrument.
o The helium consumption amounts to LESS THAN 5sccm/min.
ADDITIONAL CONSIDERATIONS
To satisfy the prior preparation of the site before installation date, the
customer must ensure the following user connections of helium and nitrogen
gas supply:
o Helium: SWAGELOK male connection, 3 mm, metric thread,
[3 m tube (10 ft) will be supplied with instrument].
o Nitrogen: SWAGELOK male connection for BRUKER's 6 mm
Teflon tube, metric thread,
[3 m tube (10 ft) will be supplied with instrument].
Ensure that an appropriate pressure control regulator is available - the
regulator must be able to supply gas in the above specified pressure range:
Nitrogen generators do not require an (external) regulator , because they have
built-in regulators. A ▇▇▇▇▇ of liquid nitrogen typically requires a single-
stage regulator (see manufacturers technical description).
If your are equipped with nitrogen from a house installation the regulator can
usually be a single-stage one, if the supply pressure is higher than the above
specified one. In comparison a high pressure nitrogen cylinder configuration
requires a dual-stage regulator.
EXHAUST VENTING REQUIREMENTS /X/
Exhaust venting is required for user safety. Health hazards include chemical
toxicity of solvents, samples and buffers used, as well as pump fluid vapor
and potential biohazards of aerosolized samples.
Most of the solvent and sample are aerosolized and vented directly through
the source exhaust. Due to this the source exhaust (via the drain bottle)
and the rough pump exhaust must be vented externally to the building and not
recirculated by the environmental control system.
The exhaust (environmental) system must be designed and controlled as
directed in conformance with all local laws (codes, ordinances and
regulations).
Page 5 of 8
Both exhaust sources must be vented separately to prevent traces of rough
pump oil from entering the source when drying gas is not flowing.
The sum of exhaust is up to 20 l/min and continuous as the instrument is on.
The exhaust system must have no backpressure at 20 l/min - ideally both
exhaust vents should be at or slightly below atmospheric pressure
(subpressure). If a subpressure is not available, the length of the tubing
from rough pump to the vent and from the drain bottle to the vent should each
not exceed 4600 mm (15 ft).
o DIVERT VALVE (to drain bottle)
Location: Source side / left side of instrument, small tube
Connection
to drain bottle: 8 mm (5/16") O plastic tubing + prepared
hose connection
Capacity: 1 to 2 l/min
o SPRAY CHAMBER EXHAUST (to drain bottle)
Location: Source side / left side of instrument, big tube
Connection
to drain bottle: 26 mm (1") O plastic tubing
Suggested tubing
to exhaust: 13 mm (1/2") O plastic tubing (Tygon)
Capacity: up to 15 l/min
o ROUGH PUMP EXHAUST
Location: Outlet mist filter of the rough pump
Suggested tubing
to exhaust: 13 mm (1/2") O plastic tubing (Tygon)
Capacity: 1 to 2 l/min
Connection to ventilation system shall be direct to building exterior or to
clean fume hod to prevent possible bay flow of contaminants - loss of
nitrogen gas may occur due to this.
SOLVENTS AND SUPPLIES /X/
The quality of the solvents and consumables greatly effects the ultimate
performance of LC/MS systems.
Many solvents and supplies suitable for typical LC applications have been
found to be unacceptable for LC/MS applications. This is particularly true
for full scan MS applications. Contamination issues should not be
underestimated.
Good references are:
o BUFFERS,
ACIDS, BASES: High quality e.g. suprapur small packs /
packages
o SOLVENTS: HPLC grade
o WATER: HPLC grade or from ultrapure water systems
Please consult the BRUKER office for detailed informations.
Page 6 of 8
TECHNICAL ASSISTANCE
If you need any assistance please call or write to
BRUKER Daltonik GmbH
▇▇▇▇▇▇▇▇▇▇▇▇▇▇▇▇▇ ▇
▇▇▇▇▇ ▇▇▇▇▇▇
▇▇▇▇▇▇▇
Phone : ▇▇▇▇-(▇)▇▇▇-▇▇▇▇-▇▇▇ Sales Department
▇▇▇▇-(▇)▇▇▇-▇▇▇▇-▇▇▇ Service Department
Fax : ▇▇▇▇-(▇)▇▇▇-▇▇▇▇-▇▇▇ Sales Department
▇▇▇▇-(▇)▇▇▇-▇▇▇▇-▇▇▇ Service Department
E-mail : ▇▇▇▇▇@▇▇▇▇.▇▇
▇▇▇▇▇▇▇▇▇@▇▇▇▇.▇▇
Internet : ▇▇▇.▇▇▇▇▇▇-▇▇▇▇▇▇▇▇.▇▇
Page 7 of 8
APPENDIX /X/
Dear customer,
for the correct and fast installation of the new esquire3000-TM- at your
site we need your confirmation of the realized site preparation in
conformance with the handed over Site Preparation Specification document.
Accompanying to the site preparation the local BRUKER office will contact you
to determine the required power cord and national plug appropriate for your
country and site.
According to your order you will need 5 or more low power cables (computer,
monitor, printer, syringe pump, hub etc.) with your national, power
appropriated and grounded plug and 1 IEC 60320 C13 connector (Picture 1) and
one power cable with a IEC 60320 C19 connector (Picture 2) on the instrument
side.
[PHOTO-DESCRIPTION TO COME]
Picture 1
national connector to power supply
Type C13 V
EN 60320/C13
max.10A
Picture 2
national connector to power supply
Type C19
EN 60320/C19
max.16A
FAX REPLY TO BRUKER SERVICE DEPARTMENT:
Hereby I/we verify that all of the above site preparations criteria have been
fulfilled.
----------------------------------
Company/Institute
---------------------------------- -----------------------------------
Address Postal Code, City
---------------------------------- -----------------------------------
Name Job Position
---------------------------------- -----------------------------------
Telephone Fax
----------------------------------
E-mail
----------------------------------- -----------------------------------
City, Date Signature/Stamp
PLEASE SEND THE STATEMENT TO:
-----------------------------------
Stamp
Page 8 of 8
The REFLEX-TM- dissipates up to 1400 ▇▇▇▇▇ (4777 BTU/hr).
The data system and additional equipment also contribute
significantly to the cooling load although the exact
amount depend on the configuration.
The heat produced in the spectrometer is blown out of the
system through a 100 mm O hole - please look up the Exhaust
Venting Requirements for more details.
ELECTRICAL AND POWER SPECIFICATION /X/
The customer is responsible for providing appropriate
electrical power and power outlets for all system
components:
The REFLEX-TM- instrument needs a three-phase high power
connection at your site with a CEE socket (IEC 60309).
Ensure at least one CEE socket for the REFLEX-TM-
instrument (permanent connection with a cable-mounting
connector, Picture 1, or better as panel-mounted socket,
Picture 2).
[PHOTO-DESCRIPTION TO COME]
Picture 1
CEE cable-mounting Connector
16A/220-415 VAC
[PHOTO-DESCRIPTION TO COME]
Picture 2
CEE panel-mounted socket
16A/220-415 VAC
The computer system includes a wide range input that can be
operated between 110 to 240 VAC +/- 10%, 50/60 Hz +/- 3 %.
The computer system can be connected through the main
internal REFLEX-TM- power distribution system or separate
power outlets. For a increased security of your stored
software data we recommended separate outlets connected to
a separate circuit or better a UPS.
Page 4 of 10
Note that the correct grounding of the electrical installation must be
guaranteed by the customer. It is inadmissible to use the neutral wire as
safety ground. The ground wire should be an isolated ground, carrying zero
current except in the case of a fault. Interruption of the protective
conductor can cause a shock hazard for the user and can damage the instrument.
ADDITIONAL CONSIDERATIONS
* Plan extra power capacity for additional equipment.
* Every equipment, like the REFLEX-TM- and the data system, should have an
own separate circuit with a particular breaker.
* If your mains voltage is unstable use an uninterruptable power supply
(UPS).
* Electromagnetic interferences, like NMRs, cellular phones and radio
transmitters may have a bad effect on system performance.
* It is recommended to install an emergency stop to interrupt the electric
circuits and other systems in a emergency case or for maintenance.
LABORATORY SUPPLY REQUIREMENTS /X/
COMPRESSED AIR / NITROGEN GAS REQUIREMENTS
The specified performance of the REFLEX-TM- contingent on a constant supply
of compressed air or nitrogen for operating the electropneumatic isolation
gate valve between the ion source and main analyzer ▇▇▇▇▇▇▇▇.
In the case of compressed air, it should be free of oil and dry with a
pressure of 4 to 6 bar (58 to 87 PSI) and should be supplied in a tube with a
1/4"-connection (1/4" SWAGELOK connection e.g.).
Compressed-air house supply or nitrogen supply are usable, or alternatively
cylinder air can be used.
Tubing should have an inner diameter of 4mm (FESTO type PU4 e.g.). The
instrument kit includes a 5000 mm (16.4 ft) long tubing.
Ensure that an appropriate pressure control regulator is available--the
regulator must be able to supply gas in the above specified pressure range.
If your are equipped with air/nitrogen from a house installation the
regulator can usually be a single-stage one, if the supply pressure is higher
than the above specified one. In comparison a high pressure nitrogen cylinder
configuration requires a dual-stage regulator.
COOLING WATER REQUIREMENTS
Cooling water is required for the two turbomolecular pumps of the REFLEX-TM-.
The flow volume rate amount to 30 l/hr at a maximum pressure of 1.5 bar. The
recommended method of obtaining the necessary, economical refrigeration up to
15 to -20 degrees C (59 to -4 degrees F) outlet temperature is to use a small
recirculating water chiller (e.g. ▇▇▇▇▇▇▇▇ TZK400 or similar). The instrument
kit includes two 5000 mm (16.4 ft) long tubings.
Page 5 of 10
EXHAUST VENTING REQUIREMENTS /X/
WARM AIR OUTLET
The heat produced in the spectrometer cabinet is blown out of the system
through a 100 mm O hole. This warm air can either just blown into the room or
directly to the outside of the building to ease the air conditioning
(system). The instrument kit includes a appropriate 5000 mm (16.4 ft) long
tubing.
ROUGH PUMP OUTLET
For the outlet of the rough pumps you either need an oil mist filter or you
have to lead it to the outside or into your exhaust air system. Tubing: 9 mm
inner O, 12 mm outer O, 5000 mm delivered with the instrument kit.
Exhaust venting could be required for user safety. Health hazards include
chemical toxicity of solvents, samples and buffers used, as well as pump
fluid vapor and potential biohazards of aerosolized samples.
Most of the solvent and sample are aerosolized and must be vented externally
to the building and not recirculated by the environmental control system.
The exhaust system should be at or slightly below atmospheric pressure
(subpressure). If a subpressure is not available, the length of the tubing
from rough pump to the vent should not exceed 5000 mm.
Connection to ventilation system shall be direct to exterior building or to
clean fume hod to prevent possible bay flow of contaminants.
A existing exhaust (environmental) system must be designed and controlled as
directed in conformance with all local laws (codes, ordinances and
regulations).
SAFETY PRECAUTIONS /X/
The standard Nitrogen laser emits high intensity radiation at 337 nm,
250-mu-J (Class IIIb laser classification). The laser radiation is invisible
to the eye but still can cause damage to it.
To safeguard the user the REFLEX-TM- is equipped with interlocks, which
prevent operation of the laser when the instrument laser cover is open.
During normal operation mode with all covers closed (i.e. Class I laser
classification) no laser radiation can be transmitted outside the
spectrometer. BRUKER does not recommend bypassing or disabling any of the
safety features by anyone other than trained BRUKER service engineers or
running the system without covers--BRUKER will not take over any
responsibility in such cases.
The spectrometer operation involves use of high voltages. The access of high
voltage cables and high voltage feedthroughs is restricted behind the side
walls of the instrument as far as possible. Accessible high voltage
connections are marked with danger signs according to regulations.
BRUKER will not take over any responsibility for any injury incurred as
result of a misappropriate use of the instrument.
Page 6 of 10
TECHNICAL ASSISTANCE
If you need any assistance please call or write to
BRUKER Saxonia GmbH
▇▇▇▇▇▇▇▇▇▇▇▇▇▇▇ ▇▇
▇▇▇▇▇ ▇▇▇▇▇▇▇
▇▇▇▇▇▇▇
Phone : ▇▇▇▇-(▇)▇▇▇-▇▇▇▇-▇▇▇ Sales Department
▇▇▇▇-(▇)▇▇▇-▇▇▇▇-▇▇▇ Service Department
Fax : ▇▇▇▇-(▇)▇▇▇-▇▇▇▇-▇▇▇ Sales Department
▇▇▇▇-(▇)▇▇▇-▇▇▇▇-▇▇▇ Service Department
E-mail : ▇▇▇▇▇@▇▇▇▇.▇▇
Internet : ▇▇▇.▇▇▇▇▇▇-▇▇▇▇▇▇▇▇.▇▇
Page 7 of 10
APPENDIX /X/
FAX REPLY TO BRUKER DALTONICS SERVICE DEPARTMENT:
Dear customer,
for the correct and fast installation of the new REFLEX-TM- at your site we
need your confirmation of the realized site preparation in conformance with
the handed over Site Preparation Specification document. Moreover we need
your informations to configure the computers for your network.
Ensure that the fax reply will be returned at least 4 weeks before instrument
installation - the BRUKER Service Team is NOT able to begin installation
without customers confirmation!
------------------------------------------------------------------------------
Hereby I/we verify that all of the below mentioned site preparations criteria
have been fulfilled:
/ / Suitable space for the instrument
/ / Electrical installation according to Site Preparation
Specification and common electrical rules
/ / Cooling water connection
/ / Compressed-air line
/ / Exhaust ventilating outlet
/ / Network Setup according to BRUKER MALDI - TOF
Computer Configuration List
-----------------------------
Company/Institute
----------------------------- ------------------------------
Address Postal Code, City
----------------------------- ------------------------------
Name Job Position
----------------------------- ------------------------------
Telephone Fax
-----------------------------
E-mail
----------------------------- ------------------------------
City,Date Signature/Stamp
PLEASE SEND THE STATEMENT TO:
Stamp
------------------------------
Page 8 of 10
GEOMETRIC TYPES OF THE REFLEX-TM- SYSTEM
(SPACE REQUIREMENTS)
Choice N(0) 1 /X/
Besides the angular version of the REFLEX-TM- System as standard
configuration you also have the choice of a linear version to accommodate the
system to the layout of your site.
Please note, that it is indispensable to determine the desired version of the
REFLEX-TM- System, because the cable laying must be performed analogous to
the desired version.
[GRAPHIC - DESCRIPTION TO COME]
Picture 3: Angular version of REFLEX System
(Standard configuration)
[GRAPHIC - DESCRIPTION TO COME]
Picture 4: Linear version of REFLEX System
(Optional configuration)
Choice N(0) 2 /X/
Appropriate to the overall dimensions of the REFLEX-TM- System you should
intend enough free space around the system for user conveniences and
maintenance access especially.
We recommend a floor space of
3500 mm x 3200 mm (LxW)
11.48 ft x 10.50 ft
at least.
Page 9 of 10
BRUKER MALDI - TOF COMPUTER CONFIGURATION LIST
CUSTOMER DATA
ADDRESS:
-----------------------------------------
-----------------------------------------
-----------------------------------------
-----------------------------------------
CONTACT PERSON: SYSTEM ADMINISTRATOR:
Mr./Mrs. Mr./Mrs.
--------------------------- ------------------------
TELEPHONE: --------------------------- ------------------------
FAX: --------------------------- ------------------------
E-MAIL: --------------------------- ------------------------
PLEASE NOTE, THAT THE STANDARD LENGTH OF NETWORK CONNECTION CABLES
DELIVERED BY BRUKER IS LIMITED TO 10 METERS. LONGER CABLES MUST BE
ORDERED SEPARATELY!
TECHNICAL DATA
(use as often as the number of computers)
/ / SUN Workstation and/or / / PC
Hostname (e.g. TOF-MS)
(if left blank, chosen by Bruker) ---------------
IP Address (e.g. 192.168.200.1)
(if left blank, a non-internet-
routeable address is chosen) ---------------
Subnetmask (e.g. 255.255.255.224)
(if left blank, no subnetting
is assumed) ---------------
Default Gateway (IP Address)
(if left blank, No default router
is assumed) ---------------
DNS IP Address (IP Address)
(if left blank, no domain server
is assumed) ---------------
DNS Domain Name (e.g. ▇▇▇▇▇▇.▇▇▇)
(if left blank, no domain name is
assumed) ---------------
IF YOU ARE RUNNING A LAN AND/OR IF YOU WANT TO CONNECT MORE THAN ONE
PC TO THE SUN, PLEASE ANSWER THE NEXT TWO QUESTIONS:
Is there a Hub for connecting your new computer to your LAN?
/ / YES or / / NO
If BRUKER should provide a Hub, what is your LAN's bandwidth?
/ / 10 MBit or / / 100 MBit
Is there something else you want to be considered for the
network setup of your new computers?
----------------------------------------------------------
----------------------------------------------------------
----------------------------------------------------------
Page 10 of 10
EXHIBIT E
ACCEPTANCE CERTIFICATE
[GRAPHIC - DESCRIPTION TO COME]
[GRAPHIC - DESCRIPTION TO COME]
CUSTOMER:
-------------------------------------------------
-------------------------------------------------
-------------------------------------------------
-------------------------------------------------
-------------------------------------------------
MEAS.
-----
TOF MASS DIFFERENTIAL PUMPING SYSTEM
ANALYZER * 260 l/sec turbomolecular pump for ion source
* 260 l/sec turbomolecular pump for analyzer
* 3 forepumps
ultimate analyzer pressure: LESS THAN OR EQUAL TO 8 x 10 TO THE POWER OF NEGATIVE 7 Torr ______
2 min. after sample change: LESS THAN OR EQUAL TO 8 x 10 TO THE POWER OF NEGATIVE 6 Torr ______
SOURCE AND * MALDI or LDI with 337 nm Nitrogen laser
OPTICS * variable, computer-controlled attenuator
REFLEX-TM- III SPECIFICATIONS & ACCEPTANCE REPORT, MARCH 1999,
VERSION NO. 9.03., PAGE SPECIFICATIONS SUBJECT TO CHANGE WITHOUT NOTICE.
I. LINEAR MALDI-TOF WITH PULSED ION EXTRACTION = PIE-TM-
MEASURED
--------
* 150 cm flight path
* positive or negative ion analysis
* ion acceleration up to +25/-20 kV ________
* MALDI mass resolution linear (FWHM):
34,000 for m/z = 1,619.82 (Bombesin) ________
* linear MALDI mass accuracy measured on mixture
of Angiotensin I, Angiotensin II, Substance P,
and Bombesin:
Internal calibration: average error LESS THAN OR EQUAL TO 50 ppm _________
External calibration: average error LESS THAN OR EQUAL TO 200 ppm _________
* linear MALDI sensitivity: S/N GREATER THAN OR EQUAL TO 10:1 for 10 fmole
at m/z = 2,465.20 (ACTH 18-39) _________
* linear MALDI mass range:
dimer of BSA-dimer detected at ~ 264 kDa _________
REFLEX-TM- III SPECIFICATIONS & ACCEPTANCE REPORT, MARCH 1999,
VERSION NO. 9.03., PAGE 2 SPECIFICATIONS SUBJECT TO CHANGE WITHOUT NOTICE.
II. OPTIONAL REFLECTRON MALDI-TOF WITH PULSED ION EXTRACTION = PIE-TM-
MEASURED
--------
* ~ 300 cm effective flight path
* patented two-stage gridless ion reflector
* positive or negative ion analysis
* ion acceleration up to +25/- 20 kV
* reflectron MALDI mass resolution (FWHM):
GREATER THAN 20,000 for m/z ~ 3,147 (Somatostatin 28) _________
* reflector MALDI mass accuracy measured on mixture
of Angiotensin II, Angiotensin I, Substance P,
Bombesin:
Internal calibration: average error LESS THAN OR EQUAL TO 10 ppm _________
External calibration: average error LESS THAN OR EQUAL TO 50 ppm _________
* reflector MALDI sensitivity: S/N GREATER THAN OR EQUAL TO 10:1 for
10 fmole at m/z = 1,047 (Angiotensin II) _________
MALDI SOURCE:
SCOUT-TM- * Multisample/Membrane Target and Observation Optics
384 * size and shape identical to microtitre plates, allows robotic handling;
* Automatic vacuum interlock
* Exchangeable targets available: smooth, 96 spots, or 384 spots;
* Computer-controlled
* High-resolution observation optics (magnification ~ 100) for color
display with large observation area, depth perception;
REFLEX-TM- III SPECIFICATIONS & ACCEPTANCE REPORT, MARCH 1999,
VERSION NO. 9.03., PAGE 3 SPECIFICATIONS SUBJECT TO CHANGE WITHOUT NOTICE.
III. ADDITIONAL OPTIONS:
------------------------
FAST(TM) / / Optional Fragmentation Analysis and Structural TOF-MS accessory
(OR PSD) * High-precision DAC computer control of
reflector voltage with ultra-stable 2nd power supply
* Automatic FAST calibration software
* Composite FAST spectrum pasting software
* FAST-FILTER(TM) parent ion preselector with resolution 150
FAST SPECIFICATION: Using 2-3 [.?.]the FAST procedure with pulsed ion
extraction using 14 voltage steps will yield at least [.?.] of the following
12 fragment ions with an avarage mass error of less than 0.4 [.?.] to column
2 from the pasted FAST spectrum peak print-out and calculate columns 4 and 5
(magnitude only).
1 Fragment 2 measured 3a exact 3b exact 4 mass 5 % error
ion mass (Da) mass (Da) mass (Da) error in Da (4/3 x 1.00)
average monoisotopic (2-3)
b2 254.3 254.2
b3 353.4 353.2
b4 481.6 481.3
b5 580.7 580.4
b6 743.9 743.5
b7 841.0 840.5
b 955.1 954.6
b11 1212.4 1211.7
b12 1327.5 1326.7
b13 1456.6 1455.7
b16 1743.9 1742.8
b21 2301.5 2300.1
REFLEX-TM- III SPECIFICATIONS & ACCEPTANCE REPORT, MARCH 1999,
VERSION NO. 9.03., PAGE 4 SPECIFICATIONS SUBJECT TO CHANGE WITHOUT NOTICE.
FAST RESULT: NUMBER n OF FRAGMENT IONS MESURED n=_______
AVARAGE MASS ERROR (SUM .?.)=_______Da
AVERAGE MASS ACCURACY (SUM .?.)=_______%
REFLEX-TM- III SPECIFICATIONS & ACCEPTANCE REPORT, MARCH 1999,
VERSION NO. 9.03., PAGE 5 SPECIFICATIONS SUBJECT TO CHANGE WITHOUT NOTICE.
CID / / Optional Collision-Induced Dissociation Accessory
(requires FAST(TM))
IV. COMPUTER SYSTEM AND SOFTWARE:
COMPUTER SUN SPARC ULTRA 5:
SYSTEM PUBLISHED SUN SPECIFICATIONS WILL NOT BE DEMONSTRATED.
* 128 MB memory
* 4.3 GB SCSI disk
* 1.44 MByte 3.5" floppy drive
* 19" color monitor
* two serial ports, one parallel port
* CD-ROM drive for software upgrades
CHECK & fill in: * printer, type:
--------------------------------
DIGITIZER 2GHz ADC (standard)
SOFTWARE * Solaris UNIX operating system, true multiuser and multitasking
* X-11 Windows and client-server computing
* TCP/IP, NFS
* Motif graphical user interface
REFLEX(TM)III SPECIFICATIONS & ACCEPTANCE REPORT, MARCH 1999. VERSION NO. 9.03.,
PAGE 6 SPECIFICATIONS SUBJECT TO CHANGE WITHOUT NOTICE.
* XMASS(TM) applications software with simultaneous
data acquisition, processing, display, plotting
* real-time acquisition optimization
* WYSIWYG plotting
* multiple views and spectral overlay
* AUTOXECUTE(TM) software for automated acquisition and
processing of MALDI-TOF data, now including unique
FUZZY-LOGIC OPTIMIZATION for optimal data quality
under automation.
OTHER ACCESSORIES DELIVERED:
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V. ACCEPTANCE COMMENTS:
------------------------------------------------------------------------------
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REFLEX(TM)III SPECIFICATIONS & ACCEPTANCE REPORT, MARCH 1999. VERSION NO. 9.03.,
PAGE 7 SPECIFICATIONS SUBJECT TO CHANGE WITHOUT NOTICE.
CHECK APPROPRIATE BOX:
The / / installation(/ / final test) is satisfactorily completed and the
/ / warranty period (/ / customer shipment) will commence.
Date:
--------------------
----------------------------- ----------------------------------
Customer (Manager) Signature Bruker Service Engineer Signature
REFLEX(TM)III SPECIFICATIONS & ACCEPTANCE REPORT, MARCH 1999. VERSION NO. 9.03.,
PAGE 8 SPECIFICATIONS SUBJECT TO CHANGE WITHOUT NOTICE.
[GRAPHIC - DESCRIPTION TO COME]
PURPOSE OF INSTALLATION:
To ensure that instruments and systems are correctly installed and
functioning as designed in the customer's facility. Correct installation is
the first step in ensuring that instruments and systems operate reliably over
an extended lifetime.
CUSTOMER RESPONSIBILITIES:
The customer should ensure that the installation site is prepared in
accordance with the specifications contained in the relevant site preparation
document and that the necessary operating supplies, consumable and usage
dependent items such as gases, vials, syringes and solvents are available. A
customer representative should be present at all times during the
installation.
INSTALLATION CHECKLIST:
DAY 1
SHIPMENT VERIFICATION
/ / Unpack and verify condition, as well as completeness, of shipment.
- If shipment is damaged or incomplete, please notify:
1. Gerd Huelso
Bruker Product Support
Tel. (49) 421/▇▇▇▇ ▇▇▇
Fax (49) 421/▇▇▇▇ ▇▇▇
Date: ----------------
name: ----------------
2. Carrier notified:
date: ----------------
name: ----------------
3. Record damaged or missing material:
-----------------------------------
-----------------------------------
-----------------------------------
PRE-INSTALLATION CHECK
/ / Verify that the site preparation kit has been correctly and fully
installed.
- Main power for mass spectrometer
1. Check power cord for specified power and proper connection.
2. Check for available outlet for syringe pump. Verify that the power
and power outlet match that of the pump and included power cord.
3. Check for available outlets for the computer, monitor and printer.
Verify that the power outlets match that of the included power
cords.
4. Check for available outlets for the HPLC system if ordered from
Bruker. Verify that the power outlets match that of those
included with the HPLC.
- Check that the gas supply lines for the instrument have been properly
installed.
1. Verify that the 6mm od Teflon tubing is connected to an approved
nitrogen source and the 5bar (75psi) pressure is available.
2. Verify that the 3mm (1/8") od stainless steel or copper tubing has
been connected an approved helium regulator and that the helium is
99.99%+ purity.
- Check that the ventilation lines for the instrument have been properly
installed.
1. Check that one end of the reinforced tubing is connected to the
ventilation.
2. Check that the 3/4" thick wall Tygon tubing is separately
connected to the customers approved exhaust ventilation system.
- Check that the customer has the required supplies, as in Site
Preparation document recommended, for complete and successful
installation and verification.
1. Solvents: water, isopropyl alcohol, acetonitrile, methanol
2. Buffers: acetic acid, etc.
3. Eppendorf pipets, 100mul and 1000mul and corresponding tips.
page 1 of 8
ESQUIRE 3000 SYSTEM INSTALLATION
/ / Install ESQUIRE 3000.
- Wheel the ESQUIRE 3000 box into the lab.
- Heave the ESQUIRE 3000 onto the table. do this with the help of two,
better four people.
- Place the rough pump under the table and connect the power cord to the
main power module of the ESQUIRE 3000.
- Connect the fore vacuum tube to the inlet of the rough pump.
- Install the oil mist filter and the oil return kit.
- Connect one end of the reinforced tubing to the outlet of the oil mist
filter, the other end should exit in the customers ventilation system
or to outside air.
- Check oil level of the rough pump.
- Connect the end of the API source exhaust tubing to the source drain
bottle. Connect the exit of the drain bottle with 3/4" (19mm) id thick
wall Tygon tubing to a secondary ventilation system. Ideally, this
connection should be directly to a clean ventilation hood or directly
to outside air. Follow all environmental regulations.
- Plug the remaining drain bottle connection with a 1/16" (1.5mm) pin.
- Flush the nitrogen gas supply line. Install the nitrogen gas filter in
the 6mm nitrogen supply line. The nitrogen flow must be straight down.
Secure gas filter with tie wraps. Connect the outlet of the gas filter
to gas inlet on the left side of the ESQUIRE 3000 using a remnant of
the Teflon tubing.
- Flush the helium gas supply line. Connect the helium gas supply to the
esquire3000 helium gas inlet on the left side using 3mm (1/8")
stainless steel or copper tubing installed during the site preparation.
If the customer uses a helium cylinder, ensure that it is properly
secured with approved stand or wall mounting hardware. Set the
pressure on the helium regulator to 80 psi (5.6 bar). Loosen the
Swagelok helium connection on the left side of the instrument. Open
the helium cylinder and post regulator valve to allow the line to
be purged with helium for several seconds. Close the post regulator
valve and tighten the helium connection.
- Verify that all instrument covers are in place. Install power cord to
left side (main power supply) of instrument and connect to customers
power.
/ / Set up computer, monitor, printer and hub.
- After computer, monitor, printer and hub are set up install control
software of the instrument, delivered with the instrument. Copy the
content of directory EQ_xxx (xxx=serial number of the instrument) of
the instrument CD-ROM to drive D:\DATA\
Copy the methods to drive D:\METHODS\
Connect the crossed twisted pair network cable (with green plugs or
labeled "crossed") from the right LAN board in the PC (the LAN board
close to the PC housing) to port S of the hub. Switch the MDI/MDI-X
switch to MDI (for crossed cable at port 8. Use the uncrossed twisted
pair network cable (with grey plugs and unlabled) to connect the hub
with the instrument. Plug the cable to port 7 of the hub and to "10
Base T" port on the left side of the instrument.
/ / Powering up the instrument.
- Turn on the main power switch on the main power supply of the
esquire3000. Switch on the power switch on the front of the
instrument. You should hear the rough pump starting and then the first
turbo pump begins to spin, then the second turbo pump.
- After powering on the instrument, the green LED of the processor system
modul is on and the yellow LED is blinking after approx. 30 seconds.
- Start the control software, open menu Options and select Vacuum
Systems... . Observe the fore and high vacuum pressures. The fore
vacuum pressure with the source open should be approximately 1-3 mbar
and the high vacuum pressure should begin to fall into the 10(-3) mbar
range.
page 2 of 8
/ / Establish the helium and drying gas pressures.
- Press Flush Helium Line in the opened Vacuum System ... window two
times to flush the helium line.
- Open any customer valving supplying the nitrogen drying gas. Where
possible set the supplying pressure from 5-7 bar (75 - 110 psi)
- Ensure that the atmospheric end of the source sampling capillary is
not capped.
- Establish flows for the API gases. Set the NEBULIZER to 80 psi. Set
the DRYING GAS flow to 12 liters/min. Verify that the gas pressures
and flow are present.
After two minutes note the read values for:
Neb. Gas: ----------psi
Dry Gas: ----------l/min
These values show if the nitrogen gas supply is without restriction if
the read values equal the set values.
- Set the NEBULIZER to 10 psi. Set the DRYING GAS flow to 4 liters/min.
Verify that the gas pressure and flow are present.
- Set the temperature of DRYING GAS to 300 DEG. C.
/ / Connect the syringe pump.
- Connect 1 1/2 ft (50 mm) of 0.005" jd (red) PEAK tubing from the
nebulizer fitting on the top of the electrospray source to the syringe
coupling.
- Fill the syringe with HPLC grade isopropyl alcohol or acetonitrile and
rinse through the tubing and nebulizer.
- Verify that the syringe pump has the proper barrel diameter (3,26 mm)
for the 500mul syringe in use.
/ / Installing the HP 1100 HPLC system if available.
- This step should have been previously coordinated with your HP
customer engineer (CE). He should be present for the first day of
installation to concurrently install the HP 1100 HPLC system.
/ / Coupling the HPLC hardware to the joint Bruker - HP Chemstation.
- Connect the second uncrossed twisted pair network cable between port 6
of the hub and the jet direct card of the HP 1100 HPLC.
- Power up the HPLC modules
- Launch the Chemstation control using the appropriate icon in: Start,
Programs, HP ChemStations.
The Chemstation control and the Bruker control software will be joiny
started.
/ / Purging the LC.
- It is important that the HPLC is purged overnight before attempting
performance verification on the esquire3000.
- In the bottles for BOTH channels A and B prepare a 50:50 mix of
water/acetonitrile.
- With the outlet of the HPLC in a waste bottle, prime each channel (%B
set to 50%) through the vacuum degasser for at least 10 min. at
5 ml/min.
- Reduce the total flow to 0.1 ml/min and allow the pump and system to
purge overnight. Make sure that enough solvent is in each of bottles A
and B. (0.1ml/min = 6ml/hr = 120ml/20hr).
/ / Setting up the esquire3000 to bakeout overnight.
- Set drying gas flow to 7#L/min. Set the nebulizer gas to 20psi.
- Set drying gas temperature to 365 DEG. C.
/ / Record system serial numbers and fill in installation documentation.
page 3 of 8
DAY 2
/ / End the purging and bakeout of the HPLC and esquire3000.
/ / Check and, if necessary, reset the helium pressure.
- In menu Options, Vacuum Systems..., switch off the helium. Wait for
10 minutes to reach the base pressure of the instrument.
- With the instrument in standby (high voltages and drying gas heater off
and gases off) carefully remove the capillary shield and cap in the
source. Temporarily cap the end of the glas capillary with a small
silicone septum. Record the fore and high vacuum pressures:
Fore Vacuum: ___________ mbar
High Vacuum: ___________ *10(-5)mbar
- Switch on the helium and if necessary adjust the helium regulator,
located on the top of the electronic cover, to get a helium pressure
6x10(-6) mbar above the base pressure. Record the high vacuum pressure:
High Vacuum: ___________ *10(-5)mbar
- Uncap the end of the capillary and replace the metal cap and shield.
Turn on the heat and gas flows (typically: 250-300 DEG. C, 7-20psi,
3-51/min). When the source has returned to temperature record the
pressures:
Fore Vacuum: ___________ mbar
High Vacuum: ___________ *10(-5)mbar
/ / Setting up initial instrument parameter.
- Place the instrument in Operate mode.
- Load the method ▇▇▇▇_▇▇▇.▇▇ in the ESQUIRE Control software.
- Fill the syringe with diluted calibration tune mix.
Tune mix / ACN: 1/10
- Connect the syringe to the nebulizer
- Start the flow of calibration tune mix at 4mul/min (240 mul/h).
- Watch for an endplate current of approximately 25-150nA indicating that
solvent has reached the end of the needle and is spraying.
- Observe the mass peaks.
SYSTEM PERFORMANCE VERIFICATION
/ / Perform auto scan calibration for all resolutions in Standard mass range
and extended mass range. Show the customer how to do this.
/ / Verify Mass Resolution in all Operational Modes and Mass Accuracy in Mode
Standard Normal.
- Produce spectra of the tuning mix including both mass spectrum and
profile spectrum in all mass ranges and resolution modes. Use 10-20
averages and rolling 2 and save some profile spectrum. Create the
directory INSTALL and use file names:
STD_NOR0.D (std/normal, 50-1200),
STD_NOR1.D (std/normal, 1200-3000),
STD_ENH0.D (std/enhanced, 50-1200),
STD_ENH1.D (std/enhanced, 1200-3000),
STD_MAX0.D (std/maximum, 50-1000),
STD_MAX1.D (std/maximum, 1000-2000),
STD_MAX2.D (std/maximum, 2000-3000),
EXTEND0.D (extended, 200-1600),
EXTEND1.D (extended, 1600-2800).
- Load the files into Bruker DA and produce a Mass List. displaying the
mass, intensity and resolution for each file and mode. Print a Profile
MS Report of each resolution mode.
/ / Verify Negative Ion Capability.
- Show the negative ion masses of the tuning mix in mode Standard Normal.
Save a profile spectrum as
NEGATIV1.D (50-1500),
NEGATIV2.D (1500-3000),
- Load the files into Bruker DA and produce a Mass List. Print a Profile
MS Report.
/ / Perform auto isolation/fragmentation calibration.
Show the customer how to do this.
(hint: this is done using standard mass range and normal resolution)
/ / Verify Monoisotopic Precursor Selection.
- Switch ICC off and set Accu Time to a proper
value.
page 4 of 8
- While in enhanced resolution mode show mono-isotopic isolation of the
1522.97 peak. Do this by displaying the 1521.97 isotope cluster with
the profile spectrum focused on mass 1522. Then isolate 1522.97. Save a
profile spectrum of isolation off (MS1523.D) and on (MSMS1523.D).
- Load the files into Bruker DA and show the capability of monoisotopic
isolation. Produce a Peak List of both files and print a Profile MS
Report.
- Demonstrate and help the customer to produce MS/MS and MS(n) spectra.
/ / Demonstrate Auto MS/MS features.
- In ESQUIRE Control load the method
▇▇▇▇▇▇▇▇.▇▇.
- Using the diluted tuning mix solution run the method. Use AUTOMSMS.D as
data file.
- Load the file into Bruker DA and show the capability of AutoMSMS. Produce
a Peak List of every different Profile Spectrum and print a Profile MS
Report.
/ / Demonstrate positive - negative switching (alternating).
- In ESQUIRE Control load the method ▇▇▇▇-▇▇▇.▇▇.
- Using the diluted tuning mix solution focus on mass 622 m/z in positive
mode.
- In mode page, activate Alternating.
/ / Verify Mass Axis Stability.
- Produce spectra of the Tuning Mix including both mass spectrum and
profile spectrum in Standard Normal mode. Use 20 averages and rolling 2
and save a profile spectrum with scan range 50-3000. Use the file name
MASSAXIS.D.
- Load the files into Bruker DA and produce a Mass List. Print a Profile
MS Report. Compare the masses with the file STD_NOR0.D and STD_NOR1.D
measured this morning.
/ / Verify Nano ESI source if available.
- Remove the syringe pump
- Switch Control Software to Shutdown
- Remove the ESI source
- Install the Nano spray source
- Change the source in Menu page to Nano
- Switch to Operate mode
- Use 20 averages and rolling 2 and save a profile spectrum. Use file
names for positive mode: NANOPOS0.D (std/normal, 50-1200), NANOPOS1.D
(std/normal, 1200-3000), Use file names for negative mode: NANONEG0.D
(std/normal, 50-1200), NANONEG1.D (std/normal, 1200-3000),
/ / Setting up the esquire3000 to bakeout overnight.
- Install the ESI source.
- Connect the HPLC to the source.
- On the HPLC, set the flow to 0.1 ml/min, 50/50 H2O/ACN.
- On the esquire3000 set drying gas flow to 7 L/min. Set the nebulizer
gas to 20psi.
- Set drying gas temperature to 365 DEG. C.
- Start the flow on the HPLC.
page 5 of 8
DAY 3
SYSTEM SENSITIVITY VERIFICATION with
ESI source
/ / Prepare performance evaluation samples for HP 1100 HPLC.
- Prepare a dilution series of reserpine. Begin with the 5ng/mul standard
(HP p/n O2423A).
- Prepare 100ml of fresh 50/50 water/acetonitrile. Use only clean, supplied
glassware and solvents.
- Transfer the 5ng/mul reserpine standard to supplied glass vials and cap.
Label the first concentration level 5ng/mul (level 1).
- All dilutions should be done with corresponding eppendorf pipets and
supplied glass vials and caps.
- Begin the dilution with 900mul. of solvent and 100mul of the 5ng/mul
standard. This second level is labeled 500pg/mul (level 2). Shake well
before proceeding.
- Make the next dilution with 900mul of solvent in a third vial and add
100mul of the level 2 standard (500pg/mul). Label this concentration
level 50 pg/mul (level 3). Shake well before proceeding.
- Make the fourth dilution with 950mul of solvent in a fourth vial and add
50mul of the level 2 standard (500pg/mul). Label this concentration
level 25pg/mul (level 4).
Shake well before proceeding.
- Finish the dilution with 800mul of solvent in a fifth vial and add 200mul
of the level 3 standard (50pg/mul). Label this concentration level
10pg/mul (level 5).
Shake well before proceeding.
- Fill two vials with 1ml of water/acetonitrile solvent for use as two
wash vials.
/ / Verify API-Electrospray Sensitivity in MS Full Scan and MS/MS Full Scan.
- Disconnect the syringe pump from the source.
- Reinstall the ESI source if not already done.
- Connect the HP 1100 HPLC to the source.
- Place level 5 (10pg/mul) in vial position 1, followed level 4 (25pg/mul,
position 2), then level 3 (50pg/mul, position 3) and then level 2
(500pg/mul, position 4). Place wash vials in position 10 and 20.
- Load method RES_MS.M.
- Run the Method RES_MS.M (MS only, full scan). Use the data file name
RES_MS.D in directory INSTALL.
- In Bruker DA load the data file and produce an extracted ion
chromatogram of mass 609. With: +0,7, -0.3. Find peaks with auto
integrate.
Report the signal to noise ratio in a processed peak list.
The 50 pg/mul peak must have a better signal to noise ratio of 10/1.
- Run the Method RES_MSMS.M (MS/MS, full scan). Use the data file name
RES_MSMS.D.
- In Bruker DA load the data file and produce an extracted ion
chromatogram of the most abundant product ion (448 m/z), Filter: All
MS/MS With: +0,7,-0,3.
Find peaks with auto integrate.
Report the signal to noise ratio in a processed peak list.
The 10 pg/mul peak must have a better signal to noise ration of 20/1.
If any difficulty is observed in meeting any of the specifications (see
specification sheet), then it is almost certain that the HPLC system should
be conditioned for an additional evening. Repeat the conditioning that was
done on the last night, except using the 50/50 water/acetonitrile that is
currently being used for the analysis.
/ / Install APCI and check the function
- Switch control software to Shutdown.
page 6 of 8
O Change source to APCI.
O Set up APCI source and bakeout for a while.
O Couple the HPLC and the syringe pump with a "T" to the APCI source.
O Fill the syringe with Tuning Mix for APCI.
O Load the Method ▇▇▇▇_▇▇▇.▇▇ in the ESQUIRE Control software.
O Set the LC flow of 50/50 water/acetonitrile at 400ul/min and set the
syringe flow of Tuning mix at 4 ul/min(240ul/h).
O Start the syringe flow and the LC flow.
O Set the corona current so to 6000nA. If necessary set the current different.
O Use the file name APCI_POS.D and load them into the Bruker DA. Produce a
Peak List. Produce a Peak List and print a Profile MS Report.
O Switch to negative mode.
O Set the corona current so to 20000nA. If necessary set the current different.
O Use the file name APCI_NEG.D and load them into the Bruker DA. Produce a Peak
List and print a Profile MS Report.
O Set the corona current to 0nA.
O Switch to positive mode.
O Stop the flow and disconnect the syringe and the "T".
// Explain Other SW features including:
O Segment editor
O LCMS-Sequence
O Deconvolution, if available
O Divert valve
O Show Quantity Analysis Program
(should be explained by application engineer)
// Make sure that all previously printed data is properly labeled, sorted and
in customer acceptance notebook. Make sure that copy is produced for product
support records.
// Copy calibration and parameter files to disk for product support.
// Copy data from subdirectory INSTALL to customers CD-ROM and to a CD-ROM
for product support.
CONTINUED SYSTEM FAMILIARIZATION
// Explain how to use the manuals (refer customer to the operational
maintenance section).
page 7 of 8
// PRODUCT SUPPORT INFORMATION:
THIS SHOULD BE FILLED OUT BY THE SERVICE ENGINEER
O Service support:
Tel.: ____________________
(Service engineer)
e-mail: ____________________
O Application support:
Tel.: ____________________
(Name)
e-mail: ____________________
O Where to order spare parts:
Tel.: ____________________
(Name)
e-mail ____________________
page 8 of 8
CUSTOMER ACCEPTANCE
// The installation is satisfactorily completed and the warranty period will
commence. All specifications due to the esquire3000 System Specifications
has been met.
-----------------------------------
COMPANY NAME
-----------------------------------
SALES ORDER NUMBER
----------------------------------
SERIAL NUMBER
----------------------------------
CUSTOMER SIGNATURE/DATE
----------------------------------
ENGINEER SIGNATURE/DATE
// List all materials (if any) that are not accompanied with this instrument:
--------------------------------------------------------------------------------
--------------------------------------------------------------------------------
--------------------------------------------------------------------------------
--------------------------------------------------------------------------------
page 1 of 7
EXHIBIT F
WARRANTY SERVICES
WARRANTY SERVICE
[GRAPHIC - DESCRIPTION TO COME]
WARRANTY SERVICE
I. SUBJECT
This Annex specifies the warranty service conditions for the following delivered
intruments:
REFLEX III MASS SPECTROMETER
ESQUIRE 3000.
II. WARRANTY TIME
Begin of warranty period: ---------------
End of warranty period: ---------------
III. DEFINITIONS
Contact time: This is the time when the customer or the user of the
instrument can contact Bruker for a unforeseeable technical
problem.
Response time: This time is defined as the time between the first contact
regarding a unforeseeable technical problem and the reaction
of Bruker in which way the problem can be solved.
Action time: This time is defined as the time to bring the instrument to
operation again. Optimization adjustments and usual
maintenance work is not included in this time. In case
further extra problems during the service work appear the
action time can be extended.
The time frame starts with the written (FAX or e-mail)
request for service of the customer or the user of the
instrument.
Waiting Time: The time the Service Engineer can not start or continue his
work because of a problem he is not responsible for. The
waiting time must be reported separate.
Waiting time van not be taken into account of Action time or
Maintenance time.
IV. WARRANTY SERVICE
- In case of a unforeseeable technical problem the customer or the user of
the instrument shall approach the service center mentioned in annex "Bruker
Service Center". During the response time Bruker will try to locate and fix
the problem via telephone, FAX or e-mail.
- In case the problem can only be solved by Bruker on site the customer or
the user have to send a written (FAX, e-mail or letter) service request to
the corresponding Bruker office with indication of the complete address
and name of the contact personnel and the order number.
- In general the user has to fill out and sign a "Declaration of
contamination" before Service or Maintenance work will be carried out.
WARRANTY SERVICE ON REQUEST
Contact times: Monday-Friday between 8.00 a.m. and 5.00 p.m.
(excluding Swiss Holidays)
Contact office: Bruker Daltonics (Switzerland).
Details see Annex D.
Response time: 2 Working days (for back-up Service)
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Action time: Max. 10 Working days or individual agreed with the
user
Waiting time: The customer is responsible to ensure that the
Bruker Service Engineer can carry out the service
work without impediments.
Waiting times must be reported on a Bruker "Service
Report" and can be charged separate.
Reported waiting times can not be counted as action
time.
Place of service: Equipment installation site
Action reporting: All service actions and the needed spare parts will
be reported on the usual Bruker "Service Report".
The "Service Report" has been signed by the Bruker
Service Engineer and the user of the instrument
when the instrument is in operation again.
If this confirmation by the user cannot be obtained
or not given in time, the accounting will be based on
the notes of the Contractor staff member.
One copy of the report stays with the instrument,
one copy stays with Bruker and one copy will be send
to the customer.
The "Service Report" is the basic for the invoice
of the used spare parts
Service confirmation: With the singed "Service Report" Bruker confirms
that the instrument is operational again. All work
carried out is professional done.
Bruker also confirms that the instrument is safe in
normal operational use after service actions.
The user confirms with the signed "Service Report"
that he agrees the work carried out and the
operational status of the instrument.
V. RETURN OF DEFECTIVE OR USED PARTS
- Usually the contractor is responsible for the return of defective or
exchanged parts to the Bruker Service Center. For parts which can not be
handcarried the corresponding user should support the contractor to ship
the parts back. Shipping costs will be covered by the contractor.
- The user is responsible for the correct and safe decontamination and
disposal of all used incidentals and sundries (e.g. pump oil, adsorbing
substance) and all other contaminated parts (e.g. P2-plate).
- All other replaced components will become the property of Bruker.
VI. TECHNICAL IMPROVEMENTS
- If considered by the Bruker staff to be advisable to improve the
reliability of the equipment related components will be replaced or
modified without additional costs for the customer. Replaced components will
become the property of Bruker.
- In case that the customer will not allow such modifications and
improvements to be made, the contractor is entitled to terminate the
service contract for the respective devices without notice.
VII. DUTIES OF THE CUSTOMER
- The customer takes responsibility that the equipment installation site
meets the respective statutory provisions and other applicable regulations.
This in particular concerns the safety measures. He has to prepare the
installation site as laid down in the SITE PREPARATION documentation and
to maintain this state.
- If not otherwise agreed the customer has to regularly clean the device and
replace the incidentals/sundries as laid down in the OPERATING and SERVICE
MANUAL.
- The customer is in charge of protecting his confidential information and
data as well as of storing his data against loss or damage. Bruker
undertakes no warranty for the loss of data on data carriers
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which might be caused during maintenance and/or service work at the
installation site or in the production facility.
- The customer provides access for the contractor staff at the stated time
to all information and facilities (including their use) which are necessary
to carry out service work on the equipment.
- The customer is responsible to ensure that service on the equipment to be
maintained or parts of it can be carried out under safe conditions.
Devices contaminated with microbiological and/or radioactive materials will
only be serviced if proof of a proper decontamination can be submitted.
(see Bruker's Safety Requirements for the return of devices and components)
- If not otherwise agreed the customer is responsible to carry out all
preventive maintenance work which is defined by Bruker.
VIII. WARRANTY AND LIABILITY
- No warranty is applicable for damages caused by carelessness, mishandling,
operating errors, malicious damage of a third party or vandalism, unforeseen
events, wars, civil disturbances etc.
- No warranty is applicable if preventive maintenance work is either not
carried out or carried out wrong or not followed by the recommended
maintenance cycles.
- If the service proves to be insufficient for reasons the Bruker is
responsible for, he commits to clear the fault free of charge or to select
the corresponding procedure for repeating maintenance free of charge.
- For the damages caused by faults of the Bruker staff during service work
the Bruker is liable.
IX. OTHER AGREEMENTS
- To become effective, reservations, collateral or other agreements need to
be confirmed in writing by the two contracting parties.
- If individual regulations of this contract should be ineffective, the
validity of all other provisions of this contract will not be affected.
The contracting parties will replace the ineffective provisions in order to
obtain the desired economic success in another manner which is in accordance
with the law. All information, data and records will be handled according to
the Data Surveillance Act, and if no longer required will be returned
immediately or destroyed upon request.
X. RELATED DOCUMENTS
MAINTENANCE PLAN REFLEX
Maintenance Plan Esquire 3000
BRUKER SERVICE CENTERS
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MAINTENANCE PLAN REFLEX
MAINTENANCE PLAN FOR BRUKER REFLEX III MASS SPECTROMETER
INTERVAL (MONTH)
----------------
ACTION NEEDED MATERIAL 1 2 6 12
------------------------------------- ----------------------------- --- --- --- ----
1. Cleaning P2-Plate X X
2. Cleaning and greasing of the X-Y- High vacuum grease NyeTorr X X
probe movement units 5200
3. Check of laser power Lasercartridge Reflex X X
4. Check of operating fluid level Leybold N62, ▇▇▇▇▇▇▇▇ P3 X X
and quality (color) of the Rotary
Vane Vacuum Pumps
5. Cleaning the laser optics X X
6. Replacing the operating fluids of Leybold N62, ▇▇▇▇▇▇▇▇ P3 X
the Rotary Vane Vacuum Pumps
7. Replacement of operating fluid ▇▇▇▇▇▇▇▇ felt replacement X
reservoir of the turbo pumps
8. Replacement of the filter of the Replacement-Filter X
dirt trap
9. Sensitivity check with the X X
prescribed standard
10. Cleaning of pressure gauges X
11. Check of cooling system, check X
the water-level, function test of
water cooling monitor
Note: The maintenance plan is currently under review!
INCIDENTALS AND SUNDRIES BRUKER RELEX III MASS SPECTROMETER
DESCRIPTION PART BRUKER PART NO.
---------------------------------- --------------------- --------------------
High Vacuum Grease NyeTorr 5200 29514
Lasercartridge Reflex 41155
Leybold Universal-Oil N62 Leybold Part Number 38357
17702
▇▇▇▇▇▇▇▇ Universal-Oil P3 ▇▇▇▇▇▇▇▇ Part Number 17234
PK001-106-T
▇▇▇▇▇▇▇▇ Operating fluid reservoir ▇▇▇▇▇▇▇▇ Part Number 19565
PM 063265-T
Replacement Filter IImvac Part Number 19037
700 154
Channel Plate 48134
Linear Detector
Channel Plate Cartridge Reflector 21255
Detector
Prevacuum sensor ▇▇▇▇▇▇ Gauge TPR 265 43258
Prevacuum sensor ▇▇▇▇▇▇ Gauge TPR 010 42663
Highvacuum sensor Cold Cathode Gauge 13321
IKR 050
Cooling Water Monitor TCW 002 8780
Note: The incidentals and sundries are currently under review!
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MAINTENANCE PLAN ESQUIRE
MAINTENANCE PLAN FOR BRUKER ESQUIRE 3000 MASS SPECTROMETER
PREVENTIVE MAINTENANCE INTERVAL
---------------------------------------------
POS DESCRIPTION 6 MONTH 12 MONTH 18 MONTH 24 MONTH
-- ---------------- ----------------------- --------- ---------- ---------- ----------
1 Rough pump Oil Exchange
2 Oil mist filter Exchange
3 Air Exhausts Cleaning
4 Instrument Diagnostic SW Tool Run
5 Source (Endcap and Cleaning
Capillary Cap)
6 Multiplier Check Exchange
7 Nebulizer Needle Check Exchange
8 Skimmer-1 Cleaning Exchange
9 Ion Gauges Cleaning
10 Air Exhausts Cleaning
11 APCI Source Nebulizer Corona Needle Check Exchange
12 Corona Needle Check Exchange
13 Off-Line Camera Adjustment Check
Nanoelectrospray
14 On-Line Check
Nanoelectrospray
Note: The maintenance plan is currently under review!
INCIDENTALS BRUKER ESQUIRE 3000 MASS SPECTROMETER
DESCRIPTION PART BRUKER PART. NO
------------------------------------- ---------------------- ---------------
Rough Pump Oil, Inland 45 HP Nr.: 6040-0834 20221
Oil mist filter Typ EMF20 Art.Nr.A462- 13450
29-000
Multiplier, Ceramic Channel Electron- Typ 7596M 21712
Skimmer-1 HP Nr.: G1946-20089 27446
ES Shipping Kit HP Nr.: G1946-60136 27281
(incl. Nebulizer needle)
APCI Shipping Kit HP Nr.: G1947-60025
(incl.
Nebulizer corona needle
Corona needle
Note: The Incidentals are currently under review!
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BRUKER SERVICE CENTERS
In case Bruker service is required for the
BRUKER BIFLEX III MASS SPECTROMETER
OR
BRUKER ESQUIRE 3000
please contact one of the following Bruker Service Center.
Bruker On-side Service Representiv
Tel: ?(On-side Service)
Fax: ?(On-side Service)
e-mail: ?@▇▇▇▇▇▇.▇▇
Bruker Daltonics GmbH
▇▇▇▇▇▇▇▇▇▇▇▇. ▇▇
▇▇-▇▇▇▇ ▇▇▇▇▇▇▇▇▇
Tel: +41-(0)1-8259-??? (Service)
Fax: +41-(0)1-8259-??? (Service)
e-mail: ▇▇▇@▇▇▇▇▇▇.▇▇
Bruker Saxonia Analytik GmbH
▇▇▇▇▇▇▇▇▇▇▇. ▇▇
▇▇▇▇▇ ▇▇▇▇▇▇▇
Tel: ▇▇▇-▇▇▇-▇▇▇▇-▇▇▇ (Service)
Fax: ▇▇▇-▇▇▇-▇▇▇▇-▇▇▇ (Service)
e-mail: ▇▇▇-▇▇▇▇▇▇▇@▇▇▇▇.▇▇
Bruker Daltonik GmbH
▇▇▇▇▇▇▇▇▇▇▇▇▇. ▇
▇▇▇▇▇ ▇▇▇▇▇▇
Tel.: ▇▇▇-▇▇▇-▇▇▇▇-▇▇▇ (Service)
Fax: ▇▇▇-▇▇▇-▇▇▇▇-▇▇▇ (Service)
e-mail: ▇▇▇▇▇▇▇▇▇@▇▇▇▇.▇▇
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