Core Modules. Each module is an independently-controlled thermal cycler fluorometer · Solid state, non-moving parts for robust performance · Incorporates four-channel optical detection · High-intensity LEDs, silicon photodetectors, and multiple filters aflow simultaneous exitation and detection of four different special bands · Starter system includes one processing block, manual, computer and accessory kit · Expand by linking up to six processing blocks to one computer system The Smart Cycler II System’s optics allow the measurement of fluorescence in four channels at the same time. High intensity LEDs and optical filters are optimized for spectral separation. The instrument’s calibration allows use of FAM or SYBR ® Green in Channel 1, Cy3 or TET in Channel 2, Texas Red (TxR) in Channel 3, and Cy5 in Channel 4. Multiplex assays allow the detection of several targets within a single sample. The use of multiple fluorescent dyes permits detection of signal in up to four channels at one time on the Smart Cycler system. Various detection chemistries can be used with the Smart Cycler optics, including TaqMan ® and Molecular Beacons probes, Amplifluor™ and Scorpion™ primers, and intercalating dyes such as SYBR ® Green.
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Core Modules. Each module is an independently-controlled thermal cycler fluorometer · • Solid state, non-moving parts for robust performance · • Incorporates four-channel optical detection · • High-intensity LEDs, silicon photodetectors, and multiple filters aflow simultaneous exitation and detection of four different special bands · • Starter system includes one processing block, manual, computer and accessory kit · • Expand by linking up to six processing blocks to one computer system The Smart Cycler II System’s optics allow the measurement of fluorescence in four channels at the same time. High intensity LEDs and optical filters are optimized for spectral separation. The instrument’s calibration allows use of FAM or SYBR SYBR® Green in Channel 1, Cy3 or TET in Channel 2, Texas Red (TxR) in Channel 3, and Cy5 in Channel 4. Multiplex assays allow the detection of several targets within a single sample. The use of multiple fluorescent dyes permits detection of signal in up to four channels at one time on the Smart Cycler system. Various detection chemistries can be used with the Smart Cycler optics, including TaqMan TaqMan® and Molecular Beacons probes, Amplifluor™ and Scorpion™ primers, and intercalating dyes such as SYBR SYBR® Green.
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Sources: Patent License Agreement (Cepheid)