OTOLITH MICROCHEMICAL ANALYSES Clause Samples

OTOLITH MICROCHEMICAL ANALYSES. Task Leader: ▇▇▇ ▇▇▇▇▇▇ (TAMUG) Participants: AZTI: Igaratza Fraile, ▇▇▇▇▇▇ ▇▇▇▇▇▇▇▇▇▇▇▇ TAMU: ▇▇▇ ▇▇▇▇▇▇ NRC: ▇▇▇▇ ▇▇▇▇▇▇ 4.1. General overview 4.2. Origin of Atlantic bluefin tuna in the North Atlantic Ocean using δ13C and δ18O in otoliths 4.1). ▇▇▇▇▇▇▇ handling followed the protocols previously described in ▇▇▇▇▇▇ et al. (2008b). Briefly, following extraction by GBYP participants, sagittal otoliths of bluefin tuna were cleaned of excess tissue with nitric acid (1%) and deionized water. One sagittal otolith from each bluefin tuna specimen was embedded in Struers epoxy resin (EpoFix) and sectioned using a low speed ISOMET saw to obtain 1.5 mm transverse sections that included the core. Following attachment to a sample plate, the portion of the otolith core corresponding to approximately the yearling periods of bluefin tuna was milled from the otolith section using a New Wave Research MicroMill system. A two-vector drill path based upon otolith measurements of several yearling bluefin tuna was created and used as the standard template to isolate core material following ▇▇▇▇▇▇ et al. (2008b). The pre-programmed drill path was made using a 500 µm diameter drill bit and 15 passes each at a depth of 50 µm was used to obtain core material from the otolith. Powdered core material was transferred to silver capsules and later analyzed for δ13C and δ18O on an automated carbonate preparation device (KIEL-III) coupled to a gas-ratio mass spectrometer (▇▇▇▇▇▇▇▇ MAT 252). Stable 13C and 18O isotopes are reported relative to the PeeDee belemnite (PDB) scale after comparison to an in-house laboratory standard calibrated to PDB.
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