Limiting Factors. Fat Red 7B, as well as the Sudan black stains, has a much greater affinity for triglycerides and cholesterol esters than it has for free cholesterol and phospholipids. Bands seen after staining with these dyes do not reflect a true quantitation of the total plasma lipids.12 For this reason it is not recommended that relative percentages of lipoprotein bands be used to calculate the total lipid content in each fraction from a total plasma lipid value. Since most laboratories routinely offer total cholesterol and triglyceride levels, this information is unnecessary. Interfering Factors: Specimens collected in heparin should not be used since heparin alters the migration patterns of the lipoprotein fractions.
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